Background: University nursing students are in a situation of affairs in which their households have fulfilled their essential interests. As a college student, those younger humans will go away from their home for impartial life. Aim: To evaluate the effect of Pender's model-based educational intervention program on promoting healthy lifestyle practices among university nursing students. Methodology: Quasi-experimental design was applied in the research; it was conducted at the Faculty of Nursing, Tanta University, and El-Gharbeya Governorate. Two tools were used for data collection. Results: there were a statistically significant relations between all items of socio demographic criteria of the studied nurses and occurrence of COVID-19 reinfection where P<0.001 except their residence, marital status, and department items. Conclusion: The educational program based on Pender's model was effective in improving the adherence level to healthy lifestyle practices among studied students through the study stages. Recommendations: Lectures, workshops, and campaigns with periodic refreshment in service training should be regularly organized to empowering undergraduate students in different faculties in order to equip them with adequate knowledge on health promotion lifestyles behaviors.