Removal of the fucose residue from the N-glycans of the Fc portion of immunoglobulin G (IgG) results in a dramatic enhancement of antibody-dependent cellular cytotoxicity (ADCC) through improved affinity for Fcγ receptor IIIa (FcγRIIIa). Here, we present the 2.2-Å structure of the complex formed between nonfucosylated IgG1-Fc and a soluble form of FcγRIIIa (sFcγRIIIa) with two N-glycosylation sites. The crystal structure shows that one of the two N-glycans of sFcγRIIIa mediates the interaction with nonfucosylated Fc, thereby stabilizing the complex. However, fucosylation of the Fc N-glycans inhibits this interaction, because of steric hindrance, and furthermore, negatively affects the dynamics of the receptor binding site. Our results offer a structural basis for improvement in ADCC of therapeutic antibodies by defucosylation.

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This research estimates emotions of university students from their BVP (blood volume pulse). Negative emotion of university students causes school dropout, which is becoming a serious problem in Japan. It is indispensable for school staffs and counselors to know when and where students have negative emotion in the campus. Since BVP signals along with emotion changes vary with personality types, we build a model dependent on personality type, to estimate student emotion from characteristics of blood volume signals. Experimental results show that the model for each personality type improves the accuracy of emotion estimation for new students. Positive or negative emotion estimated from BVP signals contributes to enhancement of campus environment by school counselors.

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