Bioactive surfaces and materials have displayed great potential in a variety of tissue engineering applications but often struggle to completely emulate complex bodily systems. The extracellular matrix (ECM) is a crucial, bioactive component in all tissues and has recently been identified as a potential solution to be utilized in combination with biomaterials. In tissue engineering, the ECM can be utilized in a variety of applications by employing the biochemical and biomechanical cues that are crucial to regenerative processes. However, viable solutions for maintaining the dimensionality, spatial orientation, and protein composition of a naturally cell-secreted ECM remain challenging in tissue engineering. Therefore, this work used soft lithography to create micropatterned polydimethylsiloxane (PDMS) substrates of a three-dimensional nature to control cell adhesion and alignment. Cells aligned on the micropatterned PDMS, secreted and assembled an ECM, and were decellularized to produce an aligned matrix biomaterial. The cells seeded onto the decellularized, patterned ECM showed a high degree of alignment and migration along the patterns compared to controls. This work begins to lay the groundwork for elucidating the immense potential of a natural, cell-secreted ECM for directing cell function and offers further guidance for the incorporation of natural, bioactive components for emerging tissue engineering technologies.

Young adults are co-residing with their parents at higher rates now than in the past, and recent research has explored the correlates of both leaving and subsequently returning to the parental home. Of relevance here, females tend to leave home earlier than their male counterparts, and research finds that drinking and drug use are also linked to residential transitions. This research note explores if substance use during adolescence and young adulthood plays a role in gender differences in home-leaving and home-returning. We find that marijuana use plays a role in both home-leaving and home-returning, with adolescent females who use marijuana the most at risk for early exits from home, and marijuana using males the most at risk for home-returning.