5-Bromo-2′-deoxyuridine (BrdU) is frequently used as a mitotic marker in studies of cell proliferation. Recent studies have reported cytotoxic effects of BrdU on neural progenitor cells in embryonic and neonatal brains in vivo and in adult tissue studied in vitro. The present study was conducted to assess whether BrdU interferes with cell proliferation and neuronal maturation in the rat adult hippocampus in vivo. BrdU effects across a wide range of doses (40 – 480 mg/kg) on cell proliferation and the population of immature neurons in the adult hippocampus were investigated using immunohistochemical labeling methods for the cell cycle marker Ki67 and a marker for immature neurons, doublecortin. BrdU did not influence cell proliferation in the dentate gyrus or the population of immature neurons observed in the adult hippocampus relative to those observed in saline treated controls. Thus, in contrast with reports of deleterious effects of BrdU in embryonic and neonatal tissue and adult tissue studied in vitro, BrdU does not appear to have cytotoxic effects on proliferating hippocampal cells or immature neurons in vivo in rats.

Yucatec Maya (YM) is an indigenous language of Mexico which displays phonemic tonal distinctions and phonemic vowel length. These features are primarily associated with the phonetic cues of pitch and duration, which are also considered the primary correlates of stress in language. Though scholars have noted the existence of stress or accent since it was first documented centuries ago, no detailed account of stress as either a separate or related entity to tone or length has been made. In this paper, I analyze the phonetic changes evident in a series of loan words into YM from Spanish, a language without tone or length distinctions, found in elicited and conversational data as an initial step in diagnosing stress patterns in YM.