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The aim of this study was to investigate the effects of calcium silicate-based products on cytotoxicity in the 3T3 fibroblast and gelatinolytic activity of matrix metalloproteinases (MMPs). 3T3 fibroblasts were incubated directly with Ortho Mineral trioxide aggregate (MTA), BioAggregate, Biodentine, MTA Plus, MTA Angelus and MTA Cerkamed for 24 hours and seven days. The cytotoxicity was determined using an MTT assay. Supernatants were collected to determine MMP-2 and MMP-9. Data were analysed using IBM SPSS 22. Seventh day extracts of Ortho MTA and Biodentine showed reduced cell viability. Specific characterization of MMPs in cell culture demonstrated that MMP-2 (62 kPa) in the cell culture supernatants by gelatin zymography showed induced expression in four out of seven groups by 3T3 cells. No MMP-9 expression was observed. The cytotoxicity of materials revealed a significant difference in cell viability between the groups on the first and seventh days. The results of this study revealed minor cytotoxic effects for Ortho MTA and Biodentine. This study suggests that endodontic sealers induced production of MMP-2. MMP-9 might be expressed in small amounts when compared with MMP-2.

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Aim: A dentist should be empowered to have knowledge of basic biological, medical, technical, and clinical sciences to distinguish the difference between normal and pathological conditions. The aim of this study was to describe the perception of dental students about competencies related to biomedical sciences in dental curriculum. Materials and methods:A 26-question questionnaire was administered to fifth-year students who were the first group to complete the new integrated dental curriculum, to measure the extent to which the students had competences with biomedical science information, which were classified as "have knowledge," "have competence," and "have familiarity" according to the Profile and competences for the graduating European Dentist-update, 2009. A Likert-type 5-point scale was used (1 = not much, 5 = a great deal) to evaluate the answers. The opinion of the students about qualification of education was also assessed with six questions.Results: Fourteen questions related to "have knowledge" (Q1-17) of biomedical science subjects were rated somewhat, two rated them much, and one scored somewhat and much as equal on the Likert scale. Four questions related to "have competence" (Q18-24) were rated much, and three were rated somewhat. Students rated "have familiarity" with "pharmacology in general medicine" and "awareness of environmental issues relevant to dental biomaterials' use" (Q 25, 26) as somewhat. Most of the students approved of integrated education (60.7%). Conclusion:While planning and developing the curriculum aims to meet the practical needs of your future dentist, the importance of training based on competence should be taken into consideration.