Maintaining a territory and being able to hunt are imperative for the success of African lion Panthera leo prides. We aimed to determine whether captiveorigin prides display similar territorial and hunting behaviour to wild lions. Behaviours and locations of two captive-origin prides and one wild pride were collected through direct observation. All prides established territories, and core areas corresponded to resource requirements. There was no evidence that pride origin affected territorial or hunting behaviour. Captive-origin prides exhibited behaviours that lead us to be optimistic about each pride's ability to establish and defend a territory successfully, and to hunt, following reintroduction.

The wild population of the African lion Panthera leo continues to decline, requiring alternate conservation programs to be considered. One such program is ex situ reintroduction. Prior to release, long-term monitoring and assessment of behavior is required to determine whether prides and coalitions behave naturally and are sufficiently adapted to a wild environment. Social network analysis (SNA) can be used to provide insight into how the pride as a whole and individuals within it, function. Our study was conducted upon 2 captive-origin prides who are part of an ex situ reintroduction program, and 1 wild pride of African lion. Social interactions were collected at all occurrence for each pride and categorized into greet, social grooming, play, and aggression. Betweenness centrality showed that offspring in each pride were central to the play network, whereas degree indicated that adults received (indegree) the greatest number of overall social interactions, and the adult males of each pride were least likely to initiate (outdegree) any interactions. Through the assessment of individual centrality and degree values, a social keystone adult female was identified for each pride. Social network results indicated that the 2 captive-origin prides had formed cohesive social units and possessed relationships and behaviors comparable with the wild pride for the studied behaviors. This study provided the first SNA comparison between captive-bred origin and a wild pride of lions, providing valuable information on individual and pride sociality, critical for determining the success of prides within an ex situ reintroduction program.

SUMMARYThere have been recent calls to develop protocols that collect unambiguous measures of behaviour using automatic techniques in conditioning experiments on magnetic orientation. Here, we describe an automated technique for recording the behaviour of Pekin ducks in a conditioning test that allows them to express unrestricted searching behaviour. Pekin ducks were trained to find hidden food in one corner of a square arena below which was placed a magnetic coil that produced a local magnetic anomaly. The trigeminal nerve was anaesthetised by injection of lignocaine hydrochloride 2-3mm caudal to the medial canthus of each eye, medial to the globe, prior to the presentation of unrewarded tests. Lignocaine-treated ducks showed no initial preference for the magnetic anomaly whereas saline-treated control ducks showed a significant preference at the same age. A second experiment was undertaken in which the trigeminal nerve was surgically severed and 2-3mm removed, and this surgery abolished the previously observed preference for the corner with the magnetic coil in a small number of ducks. These data show that Pekin ducks are able to detect and use magnetic stimuli to guide unrestricted search behaviour and are consistent with a hypothesis of magnetoreception involving a putative cluster of magnetite in the upper beak.

This document is the author's post-print version, incorporating any revisions agreed during the peer-review process. Some differences between the published version and this version may remain and you are advised to consult the published version if you wish to cite from it.1 Exploring African lion (Panthera leo) behavioural phenotypes: individual differences and correlations between sociality, boldness and behaviour.

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