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Aim: To characterize and identify vibrios present in wild and cultured juvenile snappers (Lutjanus guttatus) in northwestern Mexico. Methods and Results: Spotted rose snapper juveniles were collected at four localities in northwestern Mexico. Bacteria were isolated from external lesions, kidney, liver, and spleen from cultured and wild caught organisms. In total, 280 isolates were obtained and fingerprinted with rep-PCR (GTG5). Nearly 93AE2% of the strains belonged to the Vibrionaceae family. Species and genera identified were Photobacterium damselae subsp. damselae (76 strains), Photobacterium leiognathi (13), Vibrio sp. (24), Vibrio alginolyticus (12), Vibrio campbellii (19), Vibrio fortis (9), Vibrio harveyi (49), Vibrio ichthyoenteri (4), Vibrio mediterranei (4), Vibrio parahaemolyticus (1), Vibrio ponticus (2), Vibrio rotiferianus (22), and four potential new species. Conclusions: A wide diversity of vibrios was found in the external lesions and internal organs of both wild and cultured spotted rose snapper juveniles. In total, 12 species of vibrios and four potential new species were identified. Significance and Impact of the Study: This is the first study on the vibrios present in the spotted rose snapper and therefore might serve as a basis for future studies and diagnosis.

A high abundance of dactylogyrid monogeneans is commonly found on the spotted rose snapper Lutjanus guttatus under culture conditions. This study provides the first description of the haematology and gill histology of L. guttatus infected by dactylogyrids. The fish were infected by cohabitation with naturally infected fish. Infection levels were categorized as low (mean intensity of 22.1 parasites per fish), intermediate (100.6 parasites per fish) or high (199.8 parasites per fish). The attachment of dactylogyrids increased the erythrocyte sedimentation rate in fish with a low level of infection. Glucose was high for all three levels of infection. An increase in the infection load was positively correlated with the total leucocyte count, thrombocyte percentage ratio and the percentage of granulocytes, whereas increased infection level was negatively correlated with the leucocyte percentage ratio and the number of lymphocytes. A high infection level on the gills led to a severe proliferative epithelial response, a high number of mucous cells, a moderate increase in chloride cells, infiltrating inflammatory cells and lamellar fusion. These host responses reflect a sublethal condition and indicate a potential risk posed by dactylogyrid infection on cultured spotted rose snapper.

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