Summary SARS-CoV-2 Spike protein is critical for virus infection via engagement of ACE2 1 , and is a major antibody target. Here we report chronic SARS-CoV-2 with reduced sensitivity to neutralising antibodies in an immune suppressed individual treated with convalescent plasma, generating whole genome ultradeep sequences over 23 time points spanning 101 days. Little change was observed in the overall viral population structure following two courses of remdesivir over the first 57 days. However, following convalescent plasma therapy we observed large, dynamic virus population shifts, with the emergence of a dominant viral strain bearing D796H in S2 and ΔH69/ΔV70 in the S1 N-terminal domain NTD of the Spike protein. As passively transferred serum antibodies diminished, viruses with the escape genotype diminished in frequency, before returning during a final, unsuccessful course of convalescent plasma. In vitro , the Spike escape double mutant bearing ΔH69/ΔV70 and D796H conferred modestly decreased sensitivity to convalescent plasma, whilst maintaining infectivity similar to wild type. D796H appeared to be the main contributor to decreased susceptibility but incurred an infectivity defect. The ΔH69/ΔV70 single mutant had two-fold higher infectivity compared to wild type, possibly compensating for the reduced infectivity of D796H. These data reveal strong selection on SARS-CoV-2 during convalescent plasma therapy associated with emergence of viral variants with evidence of reduced susceptibility to neutralising antibodies.

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Findings from this review can inform the development of treatment approaches to support pregnant women with PSA/FoB.

Background: Assessment of childbirth fear, in advance of pregnancy, and early identification of modifiable factors contributing to fear can inform public health initiatives and/or school-based educational programming for the next generation of maternity care consumers. We developed and evaluated a short fear of birth scale that incorporates the most common dimensions of fear reported by men and women prior to pregnancy, fear of: labour pain, being out of control and unable to cope with labour and birth, complications, and irreversible physical damage.Methods: University students in six countries (Australia, Canada, England, Germany, Iceland, and the United States, n=2240) participated in an online survey to assess their fears and attitudes about birth. We report internal consistency reliability, corrected-itemto-total correlations, factor loadings and convergent and discriminant validity of the new scale. Results:The Childbirth Fear -Prior to Pregnancy (CFPP) scale showed high internal consistency across samples (α >0.86). All corrected-item-to total correlations exceeded 0.45, supporting the uni-dimensionality of the scale. Construct validity of the CFPP was supported by a high correlation between the new scale and a two-item visual analog scale that measures fear of birth (r >0.6 across samples). Weak correlations of the CFPP with 2 scores on measures that assess related psychological states (anxiety, depression and stress) support the discriminant validity of the scale. Conclusion:The CFPP is a short, reliable and valid measure of childbirth fear among young women and men in six countries who plan to have children. Highlights• Fear of childbirth can precede pregnancy and is associated with preferences for cesarean section among young men and women who plan to have children in the future.• Assessing fear of birth and associated factors for young adults in different countries is an important first step in understanding why some young women and men are afraid of childbirth and how this issue might be addressed.• We developed a 10-item fear of birth scale that incorporates the main dimensions of fear reported by young adults in the literature, including fear of: labour pain, bodily damage, and complications.• The Childbirth Fear -Prior to Pregnancy (CFPP) scale had high internal consistency reliability across samples, measured one underlying construct, was highly correlated with another measure of childbirth fear, and was weakly correlated with measures of depression, anxiety and stress.

This is a PDF file of a peer-reviewed paper that has been accepted for publication. Although unedited, the content has been subjected to preliminary formatting. Nature is providing this early version of the typeset paper as a service to our authors and readers. The text and figures will undergo copyediting and a proof review before the paper is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers apply.