The content of phytopathogenic fungi and the concentration of benz(a)pyrene in soil samples located near the oil refinery has been investigated. It has been found that in the vast majority of soil samples pathogenic micromicetes are represented by genera Fusarium, Rhizoctonia, Phytophthora, which have a negative effect on crops growth. The highest content of pathogenic micromycetes, amounting to10 CFU per 1 g of soil, was found in soil samples located directly on oil spill territory. It has been found that in an overwhelming number of soil samples, the concentration of benz (a) pyrene, which belongs to organic substances of grade I hazard, exceeds the MPC. The results of the study are consistent with earlier evidence that concentrations of pathogenic micromycetes and benz (a) pyrene are higher in anthropogenic-afected soils. This is due to the negative effect of oil hydrocarbons and benz (a) pyrene on useful soil microflora, and further settlement of the released ecological niche by pathogens. The company is given recommendations on the use of soil for cultivating technical crops or its exclusion from agricultural use.

The paper presents the results of a study of the effect of benzo(a)pyrene on the activity of hydrocarbon-oxidizing soil bacteria of the genus Pseudomonas and Rhodococcus. The objects of research are samples of agrosod-calcareous soil, exposed to oil pollution, located near an oil refinery with an increased concentration of benzo(a)pyrene. Studies carried out over three years from 2017 to 2019 showed that in all soil samples that are exposed to oil pollution, the concentration of benzo(a)pyrene exceeds the MPC. It was found that an increase in the concentration of benzo(a)pyrene in the soil is accompanied by the suppression of the activity of hydrocarbon-oxidizing bacteria of the genus Rhodococcus and Pseudomonas. The presence of an inverse relationship between the content of benzo(a)pyrene and the number of microorganisms was confirmed by correlation analysis carried out using Spearman’s rank correlation coefficients (ρs) and τb-Kendall. The data presented in this work can be used in organizing bioremediation of oil-contaminated agrosod-calcareous soils. In the case of a high level of soil pollution with oil products, for its effective restoration, additional support for the activity of hydrocarbon-oxidizing microorganisms is needed, including by loosening.