No abstract

Plasma N-glycan profiles have been shown to be defective in type II diabetes Mellitus (T2DM) and holds a promise to discovering biomarkers. The study comprised 232 T2DM patients and 219 healthy individuals. N-glycans were analysed by high-performance liquid chromatography. The multivariate integrative framework, DIABLO was employed for the statistical analysis. N-glycan groups (GPs 34, 32, 26, 31, 36 and 30) were significantly expressed in T2DM in component 1 and GPs 38 and 20 were related to T2DM in component 2. Four clusters were observed based on the correlation of the expressive signatures of the 39 N-glycans across T2DM and controls. Cluster A, B, C and D had 16, 16, 4 and 3 N-glycans respectively, of which 11, 8, 1 and 1 were found to express differently between controls and T2DM in a univariate analysis $$(p < 0.05)$$ ( p < 0.05 ) . Multi-block analysis revealed that trigalactosylated (G3), triantennary (TRIA), high branching (HB) and trisialylated (S3) expressed significantly highly in T2DM than healthy controls. A bipartite relevance network revealed that HB, monogalactosylated (G1) and G3 were central in the network and observed more connections, highlighting their importance in discriminating between T2DM and healthy controls. Investigation of these N-glycans can enhance the understanding of T2DM.

The rising trends of insect resistance, coupled with escalating environmental pollution from synthetic pesticides, heighten the need for a more effective and, non-hazardous agents to control insect/pests. Different aqueous extracts of Mentha spicata were screened for their phytochemical constituents and their antifeedant activities against Spodoptera frugiperda . Screening of the different aqueous extracts of Mentha spicata obtained by cold maceration revealed the presence of phenolics and tannins. The concentration of phenols and tannins in the water, glycerine, and glycerine plus water (glycerine-water) extracts were significantly different (p < 0.05). Mentha spicata water extract had a greater antifeedant activity against Spodoptera frugiperda as compared to glycerine and glycerine-water (60:40, v/v) extracts at a concentration of 5g/100 mL. The estimated % antifeedant activity recorded were 97 as against 8.21 and 49.81, respectively. Aqueous neem seed water extracts gave an estimated % antifeedant activity of 93.07 and it served as a control. Saponins were absent in all extracts and only water extracts had alkaloids present. The simple, non-hazardous, and cost-saving extraction method demonstrated could be applied in both commercial and subsistent farming to counteract the damnable effects of Spodoptera frugiperda infestation.

The rising trends of insect resistance, coupled with escalating environmental pollution from synthetic pesticides, heighten the need for a more effective and, non-hazardous agents to control insect/pests. Different aqueous extracts of Mentha spicata were screened for their phytochemical constituents and their antifeedant activities against Spodoptera frugiperda. Screening of the different aqueous extracts of Mentha spicata obtained by cold maceration revealed the presence of phenolics and tannins. The concentration of phenols and tannins in the water, glycerine, and glycerine plus water (glycerine-water) extracts were significantly different (p < 0.05). Mentha spicata water extract had a greater antifeedant activity against Spodoptera frugiperda as compared to glycerine and glycerine-water (60:40, v/v) extracts at a concentration of 5g/100 mL. The estimated % antifeedant activity recorded were 97 as against 8.21 and 49.81, respectively. Aqueous neem seed water extracts gave an estimated % antifeedant activity of 93.07 and it served as a control. Saponins were absent in all extracts and only water extracts had alkaloids present. The simple, non-hazardous, and cost-saving extraction method demonstrated could be applied in both commercial and subsistent farming to counteract the damnable effects of Spodoptera frugiperda infestation.