Streptomyces is the most frequently described genus of Actinomycetes, a producer of biologically active secondary metabolites. Indeed, the Streptomyces species produces about 70% of antibiotics and 60% of antifungal molecules used in agriculture. Our study was carried out with the goal of isolating and identifying antimicrobial secondary metabolites from Streptomyces misionensis V16R3Y1 isolated from the date palm rhizosphere (southern Tunisia). This strain presented a broad range of antifungal activity against Fusarium oxysporum, Aspergillus flavus, Penicillium expansum, Aspergillus niger, Candida albicans, Candida metapsilosis, and Candida parapsilosis and antibacterial activity against human pathogenic bacteria, including Escherichia fergusonii, Staphylococcus aureus, Salmonella enterica, Enterococcus faecalis, Bacillus cereus and Pseudomonas aeruginosa. The purification procedure entailed ethyl acetate extract, silica gel column, and thin layer chromatography. Based on 1H NMR metabolomic procedure application, also supported by the GC-MS analysis, cyclic dipeptide (l-Leucyl-l-Proline) was identified as the major compound in the bioactive fraction. In order to confirm the identity of the active compound and to have a large quantity thereof, a chemical synthesis of the cyclic dipeptide was performed. The synthetic compound was obtained with a very good yield (50%) and presented almost the same effect compared to the extracted fraction. This study indicates for the first time that Streptomyces misionensis V16R3Y1 exhibits a broad spectrum of antimicrobial activities, produced cyclic dipeptide (l-Leucyl-l-Proline) and might have potential use as a natural agent for pharmaceutical and agri-food applications.

Lobularia maritima, commonly known as sweet alyssum, is an annual ornamental halophyte widely spread along the Tunisian seashore. However, little is known about the phytochemical, antioxidant, and antimicrobial activities of Lobularia maritima. The present study aimed to investigate the potential biological properties of different parts (flowers, leaves, roots, and stems) of Tunisian L. maritima using diverse extraction methods. Extracts were then studied for their antioxidant properties, and the highest antioxidant activity was presented in the roots’ fractions. Added to this, flower, leaf, and root fractions showed interesting antimicrobial and antifungal activities against different Gram+ and Gram− bacteria and against Aspergillus ochraceus. Finally, the most active fractions (presenting the highest biological activities) were analyzed using silica gel purification and mass spectrometry coupled to gas chromatography (GC-MS) analysis, and different compounds were identified such as camphor, amide of oleic acids, tributyl acetylcitrate, betulinaldehyde, menthol, 1′-(butyn-3-one-1-yl)-, (1S, 2S, 5R), benzyl benzoate, 7-acetyl-6-ethyl-1,1,4,4-tetramethyltetralin, 2,4-heptadienal, (E,E), and nootkaton-11,12-epoxide. This work represents the first in-depth investigation of the content of bioactive compounds from Lobularia maritima. This species could potentially be a promising source of useful compounds for therapeutic applications.

The ability of Plant Growth Promoting Rhizobacteria (PGPR) to produce ammonia (NH3) is one of the key mechanisms for the growth and development of plants. Ammonia spectrophotometric quantification assay using Nessler’s reagent remains laborious and seems to be not suitable for large-scale PGP screening assay. Here, we performed a new accurate, easy, and cost-effective micro-plate protocol for large-scale ammonia quantification in bacterial supernatants as an alternative to the spectrophotometric method. In order to validate the accuracy of our innovative microplate assay, 9 bacterial strains were explored for their ability to produce ammonia using both the conventionally described assay and the newly performed micro-plate one. The ammonia 96-well microplate assay was successfully performed by scaling down the spectrophotometric methods to reduce the volume of bacterial supernatant as well as Nessler’s reagent. The reduction was estimated to 90%of the total used volume in comparison to the conventional test. The micro-test is 10-fold cheaper and 26 times faster than the conventional method. All bacterial isolates were positive for ammonia production. Bacillus inaquorsum and Bacillus mojavensis produced the highest ammonia concentration of about 371 and 370μM respectively. Furthermore, the application of the ammonia micro-plate assay reduces drastically the reagent waste and toxicity hazard of K2HgI4 (Nessler’s reagent) in the environment, thus, we can classify it as eco-friendly respecting the Green Chemistry concept according to Environmental Protection Agency (EPA). The statistical data obtained from both assays are significantly correlated (r = 0.985, R squared = 0.9329, and p <  0.001) proving the accuracy of the micro-plate assay. The proposed NH3 micro-assay is a reliable, rapid, eco-friendly, and cost-effective method to screen plant growth-promoting potential of more than 25 bacterial strains in one micro-plate. It could be an alternative for the conventional NH3 assay as a routine research tool.