Objective: This study aimed to determine the effects of deep and superficial endotracheal suctioning on hemodynamic parameters and pain level in mechanically ventilated neurosurgical patients. Patients and Methods: This prospective, randomized, controlled experimental study was conducted on 37 patients who underwent deep endotracheal suctioning and 37 patients who underwent superficial endotracheal suctioning using open endotracheal suctioning system. The arterial blood pressure, heart rate, body temperature, respiratory rate, oxygen saturation levels and pain status of the patient were compared before and after endotracheal suctioning at 1 min, 5 min and 30 min. Results: There was no statistically significant difference between the effects of deep and superficial endotracheal suctioning methods (p> 0.05). However, there was less change in systolic and diastolic arterial blood pressure and heart rates in patients who underwent superficial endotracheal suctioning before and 30 min after endotracheal suctioning (p> 0.05). Conclusion: Superficial endotracheal suctioning caused fewer changes in hemodynamic parameters and pain levels of patients compared to deep endotracheal suctioning. For this reason, nurses should first prefer the superficial endotracheal suctioning method during the suctioning practices of neurosurgery patients.