The abuse of methamphetamine (MA) is to date detected and subsequently verified through the monitoring of MA and its metabolites within biological specimens. Current approaches require complex sample purification strategies...

A novel phytase from thermophilic Geobacillus sp. TF16 was purified approximately 5-fold using ammonium sulfate precipitation and ion exchange chromatography, and determined as a single band 106.04 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Optimum temperature and optimum pH were found to be 85°C and 4.0, respectively. The enzyme is highly thermostable and V max and K m values were calculated as 526.28 U/mg and 1.31 mM, respectively. It was also found that the enzyme exhibited a broad substrate selectivity and resistance toward proteases and effectively hydrolyzed soymilk phytate. These results suggest that this study provides an alternative phytase enzyme with enhanced properties. ARTICLE HISTORY

We have studied an electrochemical detection method for the stimulants in the forensic samples using electrochemiluminescence (ECL). In this context, amphetamine type stimulant (methamphetamine (MA)) has been studied as coreactants in the [Ru(bpy)3] 2+ (where bpy is 2,2'-bipyridine) ECL system. This approach is developed based on a glassy carbon electrode modified with [Ru(bpy)3] 2+ /Nafion composite film. LoD, LoQ and linear working range for MA are studied currently.The ECL intensity was found to be concentrations over the range of 5 x 10 -8 to 2.5 x 10 -4 mol/L. LoD for MA is 1.94 x 10 -10 mol/L. The regression coefficient is 0.9931 for the experiment. Our approach was applied in different medium such as saliva and human serum to detect MA This technique is simple, rapid, selective and sensitive, and shows potential for the highthroughput quantitation of MA. the results show that the present electrochemical approach seems to provide a sensitive detection of MA in forensic applications.