Background: This study was conducted on 200 children and adult of both sexes who attended Al-Zahra Maternity and Children Teaching Hospital &Middle Euphrates Neuroscience Center in Al-Najaf province from July 2016 to July 2017 because they complaining of some symptoms that may related to meninges infection. The mean ages of suspected patients were 13.09 years for males (109), and 11.28 years for females (91). Aim of study: Identification of biochemical contents (glucose and protein level) and cell count (neutrophil and lymphocyte) of CSF among patients with meningitis. Methodology: The biochemical examination of spinal cord fluid (glucose and protein level), as well as the number of white blood cells (lymphocytes and rectangles), was conducted for the period from June 2016 to June 2017. All the biochemical tests (glucose and protein), counting the cells of the spinal cord fluid, making the direct swabs and dyeing them with chromium were done. In addition, the samples were planted with special farms for all the subjects. The average glucose level in cerebrospinal fluid of meningitis patients was 62.54 mg / dl. While the mean total protein level in CSF of meningitis patients was 27.23 mg / dl. Equivalent and lymphatic cell counts were predominant in the same spinal fluid of patients. Results: The mean level of glucose in CSF of meningitis patients was 62.54 mg/dl. The mean level of total protein in CSF of meningitis patients was 27.23mg/dl. Neutrophil & lymphocyte was the predominant cell in CSF of all suspected meningitis patients. Conclusion: The positive parameters in the analysis of CSF may be used to differentiate between bacterial and non-bacterial meningitis. Positive bacterial meningitis does not depend on the result of CSF sugar & protein only. Recommendation: Give priority to medical soon attending and laboratory analysis