No abstract

Globally, stored grain is vulnerable to pest infestation, resulting in significant economic losses for some crops. Wheat is one of the most important crops in the world. Many sucking, piercing insects infect wheat in the form of grains or flour and may produce toxic residues that are harmful to human health. The current study aimed to estimate the safe use of four stored grain insects by evaluating the potential genotoxic effects and cytotoxicity of crushed insects (T. granarium, S. oryzae, R. dominica, and T. castaneum) and their flour residues. MTT and comet assays were conducted to assess the effects of six concentrations of insect flour residues (0, 6.5, 12.5, 25, 50, and 100%) on the baby hamster kidney cell line (BHK-21). The lowest BHK-21 cell viability was noted against T. granarium (LC50% 36.42 μg/ml) followed by T. castaneum flour (LC50% 46.73 μg/ml) compared to the control (LC50% 808.2 μg/ml). Significantly high DNA comet (%) was observed in the treatments of T. castaneum flour (18.8%), S. oryzae wheat (15.6%), T. granarium (15.4%), T. castaneum (13.6%), and T. granarium wheat (13.1%). FTIR spectra of stored grain insects and their flour residues identified various functional metabolite groups, including alkynes and phenols, which could enhance cell apoptosis and genotoxicity. T. granarium, T. castaneum, and their flour residues had the highest cytotoxic and genotoxic effects on the BHK-21 cell line. The current study concludes that insect residues in flour may have cytotoxic and genotoxic effects on living cells, potentially affecting public health, particularly after consuming T. granarium and T. castaneum-infested flour. Therefore, good storage of stored grains and their products is recommended.

Digenea simplex (D. simplex), an Egyptian marine red macroalga, contains a diverse group of phytochemicals with unique bioactivities. At the same time, the synthesis of nanosuspension (NS) has received increasing interest to optimize the technological aspects of drugs. Thence, the main objective of this work was to use the chloroform extract (ChlE) of D. simplex to prepare its nanosuspension (ChlE-NS) formulation to increase its aqueous solubility, thereby improving its bioactivity. By using FTIR, GC/MS analysis, and phytochemical screening assays, the chemical profiling of ChlE was assessed. NS was prepared by the antisolvent precipitation technique using 1.5% w/v polyvinyl alcohol (PVA). A light microscope, FTIR, particle size distribution, polydispersity index (PDI), and zeta potential (ZP) measurements was used to characterize the prepared NS. Four cancer cell lines were used in the MTT experiment to investigate the anticancer potential of ChlE and ChlE-NS. An apoptotic mechanism was established using acridine orange/ethidium bromide (AO/EB) dual staining, DNA fragmentation, and increased caspase activity. ChlE and ChlE-NS were also evaluated as antioxidants using DPPH and ABTS free radical assays. The results showed that, when compared to ChlE, ChlE-NS had greater cytotoxic activity against the four cancer cell lines. However, results of antioxidant activity showed that ChlE-NS had an IC50 of 36.86 ± 0.09 and 63.5 ± 0.47%, while ChlE had values of 39.90 ± 0.08 and 86.5 ± 0.8% in DPPH and ABTS assays, respectively. Based on the results of this research, D. simplex ChlE-NS may be an effective strategy for enhancing ChlE's cytotoxic and antioxidant activities. Graphical abstract