Introduction: H. pylori is the most common human bacterial pathogen that colonizes the gastric epithelium especially in those habitual Qat chewers; it influence can absorption of glucose which is also abnormal in DM patients, its common infection in diabetic patients who have inadequate metabolic control, this study was conducted to reveal the prevalence of H. pylori among T2DM and non-DM patients and potential risk factors. Methods:A cross-sectional study was conducted in some hospitals and clinics in Ibb city, Yemen, and there were 200 patients who included in this study. Data were collected through structural intervals questionnaire and sampling information. Results:The overall prevalence of H. pylori among diabetics and non-diabetics was 39.0%. Its prevalence in diabetics was 49%, while in non-diabetics was 29%.There was strong significant association between H.pylori infections and diabetes P=0.004, H.pylori infections and Qat chewing P=0.003 and H.pylori infection and the increase in HbA1c P=0.001. Conclusion:Positive H. pylori DM patients should update their sugar level values and control the disease. Further research is highly recommended on relationship between H. pylori infections and diabetes complications (using a cohort study) enhancing personal hygiene and prevention of chewing Qat is essential to decrease the risk of infection.