The main aim of present study was to identify the seroprevalence of N. caninum infections in cattle of some districts in Wasit province using a serological test (indirect-ELISA), and confirmation of seropositive results by a molecular PCR. In this study, the blood samples and epidemiological required data were collected from 327 animals during a period of September 2015 to May 2016. The overall results were revealed that 27.22% and 12.36% of study's cattle were positives with indirect ELISA and PCR, respectively. Regarding to the epidemiological risk factors submitted for this study; the prevalence of seropositive rates was reported a statistically variable results. Among district factor, the seropositive results were 36.28%, 27.88%, 17.31% and 26.53% in Al-Azizyah, Al-Numaniyah, Al-Kut and Al-Hay districts, respectively. According to age factor, it was 18.58% and 31.78% in  3 years and  3years groups, respectively; while in sex factor, males were reported 24.53% and females 27.74%. Relating to reproductive statement factor, the positive infections were 30.99% and 21.97% in aborted and non-aborted cows, respectively. Whereas in productivity nature factor; it was 38.24% and 19.37% in dairy and beef cattle, respectively.

The study aimed to serologically detect the prevalence of Sarcocystis infection in buffaloes using the indirect enzyme-linked immunosorbent assay (ELISA) for the first time in Iraq. An overall 184 buffaloes of different ages and sexes were selected randomly from the regions of Wasit province (Iraq) from March to June (2022), and subjected to draining of venous blood under aseptic conditions. After centrifugation, the obtained sera were examined by indirect ELISA. Totally, 33.15% of study animals were positive for IgG antibodies. According to their concentrations, the ODs level showed a significant increase (P<0.0186) in mild infection (63.93%) when compared to moderate (27.87%) and strong (8.2%) infections. Subsequently, values of mild, moderate, and strong infections were 0.330 ± 0.01, 0.554 ± 0.019 and 0.912 ± 0.031 nm, respectively. Regarding age and sex factors, significantly higher positivity (P<0.0469) was shown in buffaloes aged >10 years old (57.89%); while the lowest was seen in those <1 year (13.64%). Subsequently, older buffaloes appeared significantly (P<0.0192) at higher risk (2.1685) when compared to other age groups. Females recorded an obvious increase (P<0.05) in positivity (35.95%) and risk (1.8569) in comparison with males; 19.35% and 0.5385, respectively. The results indicate that ELISA is of great value in the diagnosis of sarcocystosis.