Natural estrus induction methods were used in non receptive female rabbits for mating. Methods used were litter separation in the suckling females or presence of a female near buck cage in the non-suckling females of two differnt breeds, Baladi Red (BR) and New Zealand White (NZW). Treatments showed pronounced improvement in both breeds in terms of receptivity to males and conception rate. The NZW rabbits doe showed improvment in receptivity up to 68% in non-suckling and 73% in suckling females, while the percentages in Baladi rabbits were 72% and 79% in non-suckling and suckling ones, respectively. In addition, natrual methods tended to enhance conception rate in NZW rabbits to 74% in non-suckling and 56% in suckling females, while the corrseponding values of Baladi does were 71% and 55%, respectively. Significant decrease was observed in prolactin hormone level in response to litter separation from 42 to 6 ng/ml in NZW and from 15 to 2 ng/ml for Baladi does. However, progesterone was increased from 0.9 to 2.2 ng/ml in litter separation method and from 1.5 to 11.5 ng/ml in presence beside male cage in NZW rabbits. In Baladi does, values of progesterone hormone were increased from 0.8 to 6.7 ng/ml in litter separation and from 3.8 to 12.1 ng/ml in presence beside male cage. Estrogen levels of NZW does showed insignificant increase after litter separation and significant increase after presence beside male cage (48 to 63 pg/ml). In addition, Baladi rabbits had insignificant increase after litter separation. However, significant increase in estrogen values (from 47 to 56 pg/ml) after treatment were observed by presence beside male cage. Generally, the natural methods used to induce estrus in NZW and Baladi rabbits were successful and applicable. They can be widely used in rabbit farms instead of hormonal methods.