No abstract

<p>Plastic is a packaging materials that are widely used but has an adverse impact on the environment because it is difficult to degrade in nature. Production technology of biodegradable plastics from natural resources that have characteristic environmentally friendly has developed. Starch-based biodegradable plastic is a widely developed type because the production process is simple and the raw materials more readily available. The starch of cassava and sago has potential as a raw material of biodegradable plastic because of the availability and its characteristic. Also, to make starch as the main ingredient, plasticizers and structural strengthening materials are required to produce biodegradable plastic with excellent characteristics. The production stages of biodegradable plastics include mixing, heating, and casting. The starch-based biodegradable plastic that can apply to an environmentally friendly packaging material has an excellent opportunity developed in Indonesia.</p><p>Keywords: Starch, sago, cassava, biodegradable plastics, production technology</p><p> </p><p><strong>Abstrak</strong></p><p>Plastik merupakan bahan pengemas yang banyak digunakan namun berdampak buruk bagi lingkungan karena sulit terdegradasi di alam. Teknologi produksi plastik biodegradable atau bioplastik yang dibuat dari bahan alami dan ramah lingkungan sudah mulai dikembangkan. Plastik biodegradable berbahan dasar pati relatif lebih mudah diproduksi dan bahan baku mudah diperoleh. Pati ubi kayu dan sagu memiliki potensi sebagai bahan baku plastik biodegradable ditinjau dari ketersediaan dan karakteristiknya. Selain pati sebagai bahan utama, diperlukan pula plastisizer atau bahan pemlastis dan bahan penguat struktur untuk menghasilkan plastik biodegradable dengan karakteristik yang baik. Tahapan produksinya meliputi pencampuran, pemanasan, dan pencetakan. Plastik biodegradable berbahan dasar pati dapat digunakan sebagai bahan pengemas yang ramah lingkungan dan berpeluang besar dikembangkan.</p><p>Kata kunci: Pati, sagu, ubi kayu, bioplastik, teknologi produksi</p>

Sago starch has potential as source of flour for noodle. However, noodle made of sago starch has only been limitedly utilized due to the absence of gluten and lack of desired functional properties. Heat moisture treatment (HMT) is a promising technique for improving quality of sago noodle. The objectives of the present work were to study the effect of HMT of sago starch on its noodle quality. Four different origins of sago starch, i.e. Tuni, Ihur, Molat, and Pancasan, were treated with HMT method. HMT was performed by exposing the starch to high temperature (110oC for 16 hours) at moisture content of 25%. Sago starch was then processed into noodle. It was prepared by mixing the sago starch with binder (completely gelatinized starch and additive) into dough. The dough was pressed manually through a container with holes in the base. Noodles strains were steamed for 2 minutes and dried at 50oC in a convection drier. As the control, non-HMT sago starch was used and evaluated. Parameters evaluated were starch properties, physical strength, and cooking and sensory quality of the noodles. Analyses of variance was subjected to all parameters. Research results showed that the starch exposed to HMT changed its pasting profile from initial type A before treated to type B after treated. The noodle quality was also improved. Noodles resulted from starch treated with HMT showed higher firmness and elasticity, but they have lower stickiness compared to those of non-HMT. Less cooking loss and rehydration weight were also found, however, HMT increased cooking time of the noodles. HMT on Pancasan sago starch resulted in noodles which were preferred most by panelists. However, consumer testing is recommended to further validate consumers' preferences to the sago starch noodles. The study indicated that sago starch could be potentially used as raw material for noodles to increase the consumption of sago-based food.

Cell free supernatant containing short chain fatty acid (SCFA) resulted from fermentation of resistant starch type three (RS3) by Clostridium butyricum BCC B2571 or Eubacterium rectale DSM 17629 were investigated for their ability to inhibit proliferation and induce apoptosis of human colon cancer cell line HCT-116. HCT-116 was cultured in complete medium and after 50% confluent, incubation was continued for another 48 hours in the absence or presence cell free supernatant containing SCFA mixture at butyrate levels up to 10 mM. The study revealed that the proliferation inhibition effect was higher (>80%) on HCT-116 treated with supernatant of C. butyricum BCC B2571 than that (<70%) of HCT-116 treated with supernatant of E. rectale DSM 17629. The cells were induced to undergo apoptosis by both supernatant. The apoptosis occured through mitochondrial pathway by changing the expression of gene Bcl-2 and Bax, thus incresed the Bax/Bcl-2 ratio by more than 3.5 fold. The protein caspase-3 was increased by more than 250% in the presence of the cell free supernatant