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The flow characteristics of a single and the two side-by-side spheres immersed in a uniform flow for Re = 5 × 103 are studied qualitatively and quantitatively employing dye visualization and a particle image velocimetry. The current study focuses on the analysis of the flow behavior using instantaneous and time-mean flow data in plan-view and cross-sectional planes. Flow features and distributions of velocity vectors, V*, at the critical locations; patterns of velocity fluctuations, u* and v*; contours of sectional streamlines, ⟨ψ⟩; patterns of vorticity, ω*; and correlations of Reynolds stress, ⟨v′w′/U ∞ 2⟩ are interpreted. The gap ratios between two side-by-side spheres varying within the range of 1.0 ≤ G/D ≤ 2.50 influence the instability of flow structures substantially.