Objectives:The IgG antibodies from species of Camelidae are named heavy-chain antibodies that lack L-chains of typical antibodies. The antigen-binding site of the dromedary HCAb is located in the single-chain domain referred to as the nanobody. Nanobodies are distinguished from other conventional antibodies by their unique properties, which lead to numerous biopharmaceutical and medicinal applications. Therefore, this study aimed to isolate IgG protein by isoforms from the blood serum of Mongolia's two-humped camels and determine the contents of isomers of the antibody to enable further C. bactrianus nanobody research. Methods: IgG subclasses were separated by two different affinity chromatography steps using the ÄKTA Prime fast protein liquid chromatography method. The immunoglobulin fractions were determined using assays by the Bradford method and absorbance at 280 nm. The purity of IgG fractions was verified by the SDS-PAGE electrophoresis method. Results: Camel blood serum IgG subclasses were isolated by Protein A and G affinity chromatography columns, and the ratios of IgG1, IgG2, and IgG3 isotypes were 40.5 ± 4.5 kDa, 24.0 ± 8.0 kDa, and 35.5 ± 5.1 kDa, respectively. IgG2 and IgG3 subclass heavy chain bands on SDS-PAGE showed different molecular weights: 45 kDa and 43 kDa. Conclusions: Our findings suggest that IgG protein isotypes in the domestic Camelus Bactrianus serum that we identified were statistically different from C.Dromedarius serum.

The angiotensin-converting enzyme (ACE) inhibitory activity and antioxidant property of collagen and elastin hydrolysates, and their peptide fractions (< 5 kDa, 5-10 kDa, 10-100 kDa) were compared. The bovine raw-hide and paddywhack (Ligamentum nuchae) were used for the preparation of collagen and elastin hydrolysates, respectively. Unfractionated collagen and elastin hydrolysates (4 mg/mL) could reduce ACE activity by 61% and 58%. As a result of fractionation, ACE inhibitory activity of collagen hydrolysate was increased up to 85%, while this effect was not observed on elastin hydrolysate. Elastin hydrolysate showed intense radical scavenging abilities through 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azinobis-(3-Ethylbenzthiazolin-6-Sulfonic Acid (ABTS) assay. The collagen hydrolysate obtained from the bovine rawhide has more perspective for the bioactive food additive having ACEI activity in comparison with the elastin hydrolysate.