Abstract:There is evidence to imply that estrogen influences salivary electrolytes activity in women. Problem statement: In this study we investigated changes in sodium, potassium and inorganic phosphorus, calcium and magnesium which related to find out the precise day of ovulation. These changes might help to explain the fluctuation of ovarian hormones such as preovulatory estrogen surge. Approach: Human whole saliva was collected from 48 healthy subjects of women (age range 20-30 years) during reproductive phases of menstrual cycle were assayed using atomic absorption spectrometer and enzyme immune assay. Amongst a range of electrolytes have been identified such as sodium, potassium, inorganic phosphorus and calcium were comparatively found to be higher during ovulatory phase when compared to that of other phases. Changes were attributed to a probable hormonal outcome on salivary composition. Results: Conversely, in other phases (preovulatory and postovulatory) electrolytes activity like magnesium and calcium decreased later, declining significantly (p<0.05). The increase of electrolytes like sodium and potassium in human saliva, during ovulatory phase might be due to the circulation of steroid hormones. Conclusion: Further, the presence of particular salts influence by ovarian hormones like LH and progesterone in ovulatory saliva makes the possibility to expand a sign for spotlight of ovulation by means of noninvasive method.