Four native isolates of entomopathogenic fungi (EPF) Beauveria bassiana (B1and B2), Metarhizium anisopliae (M1 and M2 ) were isolated and evaluated against wheat aphid, Schizaphis graminum (Rondani). Three concentrations of spores suspension from each of the four fungal isolates (1×10 6 , 1×10 7 and 1×10 8 conidia) were used against adult aphid (one day old). The results showed that (B1) was the most effective according to LC50. Whereas, when testing the toxicity of crude metabolites of fungal isolates in four concentrations for each isolate, it was found that the highest effective toxin was (M1) followed by (B1), (M2) and (B2). Scanning electron microscope photographs (SEM) showed the mode of action of entomopathogenic fungi and its ability to colonize and how it infects aphid.