Cancers are a group of diseases characterized by uncontrolled growth and the spread of abnormal cells . Colon cancer (CC) is one of the most aggressive tumor that leading to death in the world In this study, an attempt was made to evaluate the effect of different combinations of 2-deoxy-d-glucose, metformin, and doxorubicin on colon cancer cells viability. Results revealed a significant decrease in colon cancer viability after the treatment with combinations of (2-DG- metformin, 2-DG – Dox , and 2-DG- metformin - Dox) at certain concentrations.