Macroinvertebrates were collected as part of two separate urban water-quality studies from adjacent basins, the Blue River Basin (Kansas City, Missouri), the Little Blue River and Rock Creek Basins (Independence, Missouri), and their tributaries. Consistent collection and processing procedures 2 Assessment of Macroinvertebrate Communities in Adjacent Urban Stream Basins, Kansas City, Missouri, 2007 through 2011 selected modeled multimetric indices are effective for comparing urban basins and for evaluation of water quality in the Kansas City metropolitan area. Description of Study Area Data from 18 macroinvertebrate sampling sites were selected and analyzed from urban basins in the Kansas City, Mo., metropolitan area and adjacent counties (fig. 1, table 1). Seven sites were located in the Blue River Basin, including five sites on the main-stem Blue River and one site each on Indian Creek and Brush Creek, tributaries to the Blue River. Eight sites were located in the Little Blue River Basin, including three sites on the main-stem Little Blue River and five sites located on tributaries to the Little Blue River (Adair Creek, East Fork Little Blue River, Crackerneck Creek, Spring Branch Creek, and Burr Oak Creek). Rock Creek, located almost entirely within Independence city boundaries between the Blue River and Little Blue River Basins, was included in analyses with the Little Blue River sites. Two rural sites that are relatively free from development, located outside the Kansas City metropolitan area in adjacent Ray and Cass Counties, were selected for comparison (fig. 1). descriptors include environmental perturbation, environmental disturbance, urbanization, urban gradient, and other similar terminology used with small distinctions or interchangeably in previous studies cited and in the literature for macroinvertebrate studies in urban streams. Urban intensity as used in this study should be interpreted broadly with the terminology used in the references cited.