A key aspect of bacterial survival is the ability to evolve while migrating across spatially varying environmental challenges. Laboratory experiments, however, often study evolution in well-mixed systems. Here we introduce an experimental device, the Microbial Evolution and Growth Arena (MEGA) plate, in which bacteria spread and evolve on a large antibiotic landscape (120x60cm), allowing visual observation of mutation and selection in a migrating bacterial front. While resistance increases consistently, multiple coexisting lineages diversify both phenotypically and genotypically. Analyzing mutants at and behind the propagating front, we find that evolution is not always led by the most resistant mutants; highly resistant mutants may be trapped behind more sensitive lineages. The MEGA-plate provides a versatile platform for studying microbial adaption and a direct seeing-is-believing visualization of evolutionary dynamics.

Abstract. We study the structure of social networks of students by examining the graphs of Facebook "friendships" at five American universities at a single point in time. We investigate each single-institution network's community structure and employ graphical and quantitative tools, including standardized pair-counting methods, to measure the correlations between the network communities and a set of self-identified user characteristics (residence, class year, major, and high school). We review the basic properties and statistics of the pair-counting indices employed and recall, in simplified notation, a useful analytical formula for the z-score of the Rand coefficient. Our study illustrates how to examine different instances of social networks constructed in similar environments, emphasizes the array of social forces that combine to form "communities," and leads to comparative observations about online social lives that can be used to infer comparisons about offline social structures. In our illustration of this methodology, we calculate the relative contributions of different characteristics to the community structure of individual universities and subsequently compare these relative contributions at different universities, measuring for example the importance of common high school affiliation to large state universities and the varying degrees of influence common major can have on the social structure at different universities. The heterogeneity of communities that we observe indicates that these networks typically have multiple organizing factors rather than a single dominant one.

Classical theory shows that large communities are destabilized by random interactions among species pairs, creating an upper bound on ecosystem diversity. However, species interactions often occur in high-order combinations, whereby the interaction between two species is modulated by one or more other species. Here, by simulating the dynamics of communities with random interactions, we find that the classical relationship between diversity and stability is inverted for high-order interactions. More specifically, while a community becomes more sensitive to pairwise interactions as its number of species increases, its sensitivity to three-way interactions remains unchanged, and its sensitivity to four-way interactions actually decreases. Therefore, while pairwise interactions lead to sensitivity to the addition of species, four-way interactions lead to sensitivity to species removal, and their combination creates both a lower and an upper bound on the number of species. These findings highlight the importance of high-order species interactions in determining the diversity of natural ecosystems.