The interface pressure between the residual limb and prosthetic socket has a significant effect on the amputee’s mobility and level of comfort with their prosthesis. This paper presents a socket interface pressure (SIFP) system to compare the interface pressure differences during gait between two different types of prosthetic sockets for a transtibial amputee. The system evaluates the interface pressure in six critical regions of interest (CROI) of the lower limb amputee and identifies the peak pressures during certain moments of the gait cycle. The six sensors were attached to the residual limb in the CROIs before the participant with transtibial amputation donned a prosthetic socket. The interface pressure was monitored and recorded while the participant walked on a treadmill for 10 min at 1.4 m/s. The results show peak pressure differences of almost 0.22 kgf/cm2 between the sockets. It was observed that the peak pressure occurred at 50% of the stance phase of the gait cycle. This SIFP system may be used by prosthetists, physical therapists, amputation care centers, and researchers, as well as government and private regulators requiring comparison and evaluation of prosthetic components, components under development, and testing.