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The influence of various environmental factors on amylase production has been investigated, using a bacterial isolate (No. 23) classified as Bacillus subtilis.Favorable growth and amylase production occur on a medium consisting of autoclaved wheat bran moistened with dilute phosphate buffer. Concentration and age of the inoculum have little influence on amylase production, nor do repeated serial transfers in a fluid wheat bran medium enhance production. At 37°C ., with a ratio of 1 part wheat bran to 2.5 parts liquid, maximum amylase production results after 48-hour incubation.Maximum amylase per unit of bran is produced when a ratio of 1 part bran to 1.75 parts liquid is used as medium.However, enzyme yields over the range of ratios employed indicate that amylase production is regulated primarily by the concentration of nutrient. The amylase resulting from the growth of selected isolate 23 has a starch dextrinization

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