22The oomycete pathogen Phytophthora cactorum causes crown rot, a major disease of 23 cultivated strawberry. We report the draft genome of P. cactorum isolate 10300, cultured 24 from symptomatic Fragaria x ananassa tissue. Our analysis revealed that there are a large 25 number of genes encoding putative secreted effectors in the genome, including nearly 200 26 RxLR domain containing effectors, 77 Crinklers (CRN) grouped into 38 families and 27 numerous apoplastic effectors, such as phytotoxins (PcF proteins) and necrosis inducing 28 proteins. As in other Phytophthora species, the genomic environment of many RxLR and CRN 29 genes differed from core eukaryotic genes, a hallmark of the two-speed genome. We found 30 genes homologous to known Phytophthora infestans avirulence genes including Avr1, Avr3b, 31 Avr4, Avrblb1 and AvrSmira2 indicating effector sequence conservation between Phytophthora 32 species of Clade 1A and 1C. The reported P. cactorum genome sequence and associated 33 annotations represent a comprehensive resource for avirulence gene discovery in other 34 Phytophthora species from Clade 1 and will facilitate effector informed breeding strategies in 35 other crops. 36 37 38 39 40 The oomycetes are a diverse class of eukaryotic microorganisms that include pathogens of plants, 41 animals and fungi (Simão et al. 2015). The causal agents of plant diseases are well represented in 42 this phylogenetic class, with over 60% of known oomycetes characterised as plant pathogens 43

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