This work compares the phenolic contents and the total antioxidant capacity of the 36 most popular Brazilian foods submitted to aqueous extraction or in vitro digestion. The purpose was to evaluate the extent by which digestion differs from the simple aqueous extraction procedures of several food matrices. After in vitro digestion, cereals, legumes, vegetables, tuberous vegetables, chocolates and fruits showed higher phenolic contents and higher antioxidant activities than those obtained by aqueous extraction. Contrarily, the digestion caused a reduction in the phenolic contents and antioxidant activities of beverages (red wine, coffee and yerba mate). Our results suggest that the phenolics of food groups with solid and complex matrix are protected against enzymatic action and alteration in pH during the digestion, what does not occur in liquid food matrices such as the beverages. This fact would overestimate the antioxidant activities of beverages submitted solely to aqueous extraction.
In this study, the antioxidant properties and the polyphenolic contents of raw and cooked pinhão seeds were evaluated. Four methods were used to evaluate the antioxidant activity: 1,1-diphenyl-2picrylhydrazyl free radical scavenging (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid scavenging (ABTS), ferrous ions chelating activity and lipid peroxidation inhibition (LPO). The EC 50 values (extract concentration producing 50% antioxidant effect), in μg/ml extract for raw and cooked extracts yielded respectively: DPPH, 870.7±30.8 and 112.6±5.4; ABTS, 170.7±7.8 and 52.1±2.1; ferrous ions chelating activity, 1,719±84.8 and 761±77.1; and LPO, 55.9±4.7 and 53.8±4.5. The improvement in the antioxidant activity in the cooked seeds can be attributed to the migration of polyphenolic compounds from coat to seed during cooking (5.92 ± 0.09 μg/mg and 24.06 ± 1.3 μg/mg in the raw and cooked seed extracts, respectively). Both raw and cooked seeds presented elevated amounts of catechin, 17.49±0.06 and 21.08±0.09 mg/100 g of seed, respectively.
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