Genetic approaches have provided evidence that DNA endjoining problems serve an essential role in neuronal survival during development of mammalian embryos. In the present study, we tested whether the DNA repair enzyme, DNA dependent protein kinase, plays an important role in the survival of cerebral cortical neurons in mice. DNA-PK is comprised of a DNA-binding subunit called Ku and a catalytic subunit called DNA-PKcs. In mice with the scid mutation, DNAPKcs is truncated near the kinase domain, which causes loss of kinase activity. We compared the spatial and temporal aspects of neuronal cell death in scid versus isogenic wildtype embryos and found a significant increase in dying cells in scid mice, as assessed by nuclear changes, DNA fragmentation and caspase-3 activity. Additional biochemical and immunocytochemical studies indicated that of several DNA repair enzymes investigated, only PARP was increased in scid mice, possibly in response to elevated DNA strand breaks. Cell Death and Differentiation (2001) 8, 245 ± 255.

The Meguma group of lithic greywacke, feldspathic quartzite, slate siltstone, and argillite is Early Ordovician or older in age and has undergone both regional and contact metamorphism. Both types of metamorphism have resulted in recrystallization and locally in orientation of newly formed minerals. Metasomatism and retrogressive metamorphism are subordinate and only locally important. Regionally metamorphosed rocks are divided into greenschist and almandine–amphibolite facies, although some assemblages cannot be assigned with certainty. Locally, biotite and garnet isograds are mappable within the greenschist zone.Relationships between regional metamorphism and structural elements (folding) show that deformation preceded regional metamorphism. Intrusion of granitic rocks has produced a zone of contact metamorphism (hornblende–hornfels facies) that is superimposed upon regional greenschist facies rocks, which shows that granite emplacement occurred after the regional grade was reached. Gold–quartz veins are confined to areas lying in the greenschist zone of regional metamorphism, which suggests that the almandine–amphibolite zone is not favorable.

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