The aim of this study was to evaluate eight plants extract for porcine pancreatic lipase inhibition, characterizing biochemically the extract with high inhibitory activity and its effects on preventing weight gain in female rat fed a high-fat diet (HFD). In vitro pancreatic lipase inhibition was carried out in ρ-nitrophenyl-laurate substrates and a double-reciprocal plot was used for inhibition mechanism identification. In vivo experiments, female rats were fed with a standard diet or high fat diet (HFD), HFD+orlistat, HFD+22.5 mg/ml GT, and HFD+112.5 mg/ml GT. Feed intake, weight body gain, fecal lipid excretion and biochemistry parameters were analyzed. Camellia sinensis extract had the highest inhibitory lipase activity (76.65 ± 2.04%) with a non-competitive inhibition. C. sinensis administration, equivalent to 112.5 mg/ml, promoted weight loss, while 22.5 mg/ml increased fecal excretion of lipids in 31.41%. C. sinensis extract is certainly a promising alternative for preventive obesity treatment, since biochemical parameters analyzed showed significantly, reduction in the serum triglycerides levels and significantly decreased the LDL-cholesterol basal levels when compared with animals that did not receive a fat diet.

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The aim of this work was to evaluate the use of different agro-industrial residues for the production of xylanase by Aspergillus sp. ART 100.1, as well as analyzing the biochemical properties of the enzyme. Agroindustrial residues malt bagasse, pineapple crown, açaí bagasse and soybean husk present in large quantities in the Tocantins region were used to evaluate the production of xylanase. Cultivation conditions for xylanase production were evaluated in submerged and solid-state cultivation. The highest production of xylanase in submerged cultivation was obtained using soybean husk residue (23.60 U/mL), while, for solid-state cultivation conditions, the highest production of xylanase was obtained with malt bagasse (110.00 U/g). The effect of additives to the culture medium was also evaluated, with the best result for the use of the xylose additive in the pineapple crown in solid-state cultivation. The enzyme produced in solid-state cultivation was characterized in terms of pH and temperature. The optimum activity pH was observed at 5.0 and for temperature at 55 °C. The xylanase was stable in a pH range between 4 and 5 and retained 50% of its activity at 45 °C after 110 minutes. The Aspergillus sp. ART 500.1 presents potential for the production of xylanase using agro-industrial residues, enabling the development of bioprocesses for the scaling of production.

Recall é um termo inglês, originário dos Estados Unidos, e comumente utilizado no ordenamento jurídico brasileiro para proteger o consumidor do fornecedor de bens e serviços. Em português, significa “voltar”. A descoberta de impurezas de nitrosaminas em medicamentos levou a Food and Drug Administration (FDA) e outros agências reguladoras a realizar uma análise detalhada desse contaminante em insumos farmacêuticos ativos (IFAs) e medicamentos afetados. Nesse viés, o objetivo desse estudo foi realizar uma revisão de literatura acerca do recall de substâncias medicamentosas e produtos farmacêuticos, devido à presença de impurezas de nitrosaminas. Trata-se de uma pesquisa qualitativa, que foi realizada através de revisão bibliográfica da literatura. Através desta pesquisa conclui-se que a formação de impurezas de nitrosaminas pode ser evitada selecionando reagentes, catalisadores e solventes adequados na fabricação de substâncias farmacêuticas e, sempre que esses contaminantes forem identificados em produtos farmacêuticos, acima dos limites estabelecidos pela legislação, os mesmos devem ser inutilizados e os lotes recolhidos pelas indústrias.