No abstract

Strawberries (Fragaria × ananassa Duch. cvs. Everest, Elsanta) were grown in a tunnel covered with two films, which were distinguished in their ultraviolet transparency, as well as under open-field conditions. One applied film was not transparent for UVB radiation, and the second film transmitted 70% of UVB radiation. During the present study, the nutritional value and quality parameters of the fruits were evaluated. Strawberries were UV-unresponsive in view of the content of ascorbic acid and sum parameters like total anthocyanins and antioxidant capacity measured with TEAC (trolox equivalent antioxidant capacity), ORAC (oxygen radical absorbance capacity) and total phenols. These parameters were mainly affected by sampling date and cultivar. However, HPLC analysis showed that individual phenolics were affected in the absence of UV radiation. The content of the anthocyanin cyanidin 3-glucoside and the flavonols quercetin 3-glucuronide and kaempferol 3-glucoside was decreased in the fruits grown under UV blocking film compared to open-field grown strawberries. By means of the UV transparent film the content of the mentioned flavonoids could be enhanced up to similar amounts like in open-field grown strawberries. All other phenolics were not consistently affected by UV radiation. This result was independent of cultivar.

The site effect of five locations from north (Stjørdal, Norway, 63 • 36 N) to south (Ancona, Italy, 43 • 31 N) was evaluated in strawberry regarding yield performance, fruit quality, length of fruit developing time from anthesis to harvest start and length of the harvest season. Cv. Elsanta was grown at all sites while cv. Korona was cultivated in north and central Europe and cv. Clery in central and south Europe. Yield was more affected by seasonal and growing conditions than by latitude. Anthesis was delayed as influenced by cultivar up to 58 days from south to north and was nearly maintained until harvest start. Duration of fruit development was negatively related to daily mean temperature and increased with higher latitude. 29-34 days were required from anthesis to harvest start for cv. Elsanta, 29-36 for cv. Korona and 27-38 for cv. Clery. Corresponding GDD values (growing degree days; 3 • C base temperature) were independent from latitude and accounted to 334-355 for cv. Elsanta, 301-385 for cv. Korona and 320-434 for cv. Clery. Daily mean temperature decreased about 2 • C from south to north during anthesis to harvest start which induced a calculated 5.2 days longer fruit development period in the north. From harvest start to harvest end, GDD values varied for all cultivars between 297-402 GDD showing no influence of the latitude. However, simple summing of GDD values do not correctly describe the time of fruit development (from anthesis to the ripe fruit) for the whole fruiting period of a plant. GDDs for individually tagged flowers increased notably from the first third to the last third of the developing period due to increasing temperature as the season proceeded. The fruit quality standards dry matter, soluble solids and titratable acidity were influenced by latitude giving northern sites in general the highest values. Fruits grown at the southern sites were redder compared to those of the north.

The increasing use of biological control agents (BCAs) against Botrytis cinerea in strawberry raises the question of whether there are any undesirable impacts of foliar applications of BCAs on nontarget microorganisms in the phyllosphere. Therefore, our objective was to investigate this issue within a field study. Strawberry plants were repeatedly sprayed with three BCAs-namely, RhizoVital 42 fl. (Bacillus amyloliquefaciens FZB42), Trianum-P (Trichoderma harzianum T22), and Naturalis (Beauveria bassiana ATCC 74040)-to suppress Botrytis cinerea infections. Microbial communities of differentially treated leaves were analyzed using plate counts and pyrosequencing and compared with the microbial community of nontreated leaves. Plate count results indicate that the applied Bacillus and Trichoderma spp. survived in the strawberry phyllosphere throughout the strawberry season. However, no significant impacts on the leaf microbiota could be detected by this culture-dependent technique. Pyrosequencing of internal transcribed spacer ribosomal RNA and 16S RNA sequences revealed a change in fungal composition and diversity at class level after the introduction of T. harzianum T22 to the phyllosphere, whereas the bacterial composition and diversity was not affected by either this Trichoderma preparation or the other two BCAs. Our results suggest that pyrosequencing represents a useful method for studying microbial interactions in the phyllosphere.

The effect of four different growing locations from Stjørdal, Norway (63 • 36 N) to Conthey, Switzerland (46 • 12 N) on the composition of bioactives in strawberry (Fragaria x ananassa Duch.) of three genotypes (cvs Clery, Elsanta and Korona) was evaluated. Principal component analysis (PCA) was used to characterize differences in bioactives between genotypes within a location. Despite harvest period and year-to-year effects, a clear clustering related to the effects of the cultivars was shown. Furthermore, PCA was able to separate locations within a cultivar due to differences in bioactive compounds and this effect was stronger than local variations by temporary weather changes or even year-to-year effects. As a result, the northern samples had principally lower anthocyanin amounts than the southern ones and, moreover, distribution of individual anthocyanins was different. In contrast, the content of vitamin C and the antioxidant capacity (TEAC, ORAC, total phenols) was generally increased with higher latitudes calculated on a fresh weight basis. Results of proanthocyanidins and individual polyphenols detected by high performance liquid chromatography indicated that the higher contents were mainly due to tannin-related components. For all these observations, cultivars responded in general similarly. A North-South division was identified but no clear trend towards latitude could be recorded, mainly since one major impact factor for the biosynthesis of phenolics and ascorbic acid is temperature, which is altering not only by latitude, but also by the shift of the harvest period and the current weather conditions prior to harvest. In conclusion, genetic effects were generally stronger than environmental effects.