containing flaxseed, sesame seeds and their oils on non-alcoholic fatty liver disease (NAFLD) in rats, from some nutritional and biochemical parameters, in addition to estimate the chemical composition and fatty acids of the two types of the tested seeds. Fifty-four adult male Albino rats (Sprague Dawley strain), were used in this study and divided into two main groups. The first main group (6 rats) fed on basal diet, as a control negative group. The second main group (48 rats) fed on high fat diet (HFD) for 6 weeks to induce non-alcoholic fatty liver disease, after confirmation rats were randomly assigned to eight equal subgroups: Subgroup (1): fed on high fat diet (HFD) and used as a control positive group (+ve)1. Subgroup (2): fed on medium fat diet (MFD) and used as a control positive group (+ve)2. Subgroup (3): fed on (MFD), replace (10% sheep tallow with 10% flax seeds oil). Subgroup (4): fed on (MFD), replace (10% sheep tallow with flax seeds which provided the diet with 10% oil). Subgroup (5): fed on (MFD), replace (10% sheep tallow with 10% sesame oil). Subgroup (6): fed on (MFD), replace (10% sheep tallow with sesame seeds which provided the diet with 10% oil). Subgroup (7): fed on (MFD), replace (10% sheep tallow with 5% flaxseeds oil and 5% sesame oil). Subgroup (8): fed on (MFD), replace (10% sheep tallow with sesame seeds which provided the diet with 5% oil and flax seeds which provided the diet with 5% oil). The experiment lasted for 4 weeks. The results indicated that, treating NAFLD rats with MFD containing (10% flaxseeds oil, flaxseeds which provided the diet with 10% oil, 10% sesame oil, sesame seeds which provided the diet with 10% oil, 10% (flaxseeds oil and sesame oil) and (sesame and flaxseeds which Samar El-Sayed Mohamed (30) http://ejos.journals.ekb.eg-Vol (8) N (27) July 2020 provided the diet with 10% oil) led to significant decrease in body weight gain %, organs weight%, peritoneal fat pad PFP /body weight % PFP, leptin, glucose, lipid profile including (cholesterol, triglycerides, LDL-c and VLDL-c), liver enzymes including (AST, ALT and ALP), while these treatments induced significant increase in feed intake, HDL-c and antioxidant enzymes including (GSH-GPx, SOD and CAT). The medium fat diet MFD alone or MFD containing the tested seeds and their oils improved the nutritional and biochemical parameters in non-alcoholic fatty liver disease, especially the group fed on MFD containing the combination of flaxseeds and sesame seeds which provided the diet with 10% oils, followed by the group fed on MFD containing 10% combination of these oils. The medium fat diet MFD which contain the combination of flaxseeds and sesame seeds, or their oils improved the health status of rats suffering from non-alcoholic fatty liver disease