No abstract

The aim of study was to investigate the effect of corpus luteums (CL) size that detected before embryo transfer on conception in recipient Holstein cows. The recipient cows utilized had at least one birth and aged 3-8 years old. PGF2α was injected 2 times space 14 days apart to cows and 87 cows which show estrous symptoms were determined as recipient. Fresh embryos were transferred after estrous in 6 th -8 th days. Ovaries were examined by ultrasound before transfer and CL sizes were measured. A scale of '++++' were set as a result of measurements. Recipient cows were into 3 groups. The first group was named as CL2+ (n=25) and consisted of cows with '++' (≥15-20< mm) size CL. The second group was named as CL3+ (n=52) and consisted of cows with '+++' (≥20-25< mm) size CL. The third group was named as CL4+ (n=10) and consisted of cows with '++++' (≥25 mm) size CL. The embryos were transferred to the uterine horn on the side of the CL (ipsilateral). Pregnancy rate was respectively 36%, 46.2% and 40% for groups. No statistically significant difference was found between groups in terms of the effect of CL on conception (P>0.05). As a result, it was concluded that the size of the corpus luteum has no effect on the conception of cows utilized as recipients in embryo transfer.

The aim of the study, the relationship between coagulation parameters and embryonic loss in embryo transferred (ET) cows and heifers wereevaluated. The animal material of this study consisted of 19 cows and 19 heifers on farms located in East Mediterranean Agriculture Research Institute in Adana. Blood samples were collected before the ET application from the recipient cows and heifers. Coagulation parameters measured included prothrombin time (PT), activated thromboplastin time (APTT), fibrinogen, thrombin time (TT), anti-thrombin-III (AT3) and D-dimer using by Sysmex ® CA-7000 and activated protein C resistance, protein C and protein S using by Sysmex CS-5100. The pregnancy rate was found 5/19 (26.3%) and 5/19 (26.3%) in cows and in heifers, respectively (P>0.05). The embryonic mortality ratio in cows was 60% (3/5) and in heifers it was 40% (2/5). The differences of D-dimer levels between pregnant and non-pregnant animals were significant (P<0.05).The APTT levels between the groups with pregnancy and embryonic loss were significantly different (P<0.05). As a result, it was determined that there is a relationship between plasma D-Dimer levels and embryonic loss in cows that were transferred embryo.To the best of authors' knowledge, this is the first study reporting the relationship between coagulation parameters and embryonic loss in ET cows and heifers.

Bu çalışmada, östrus senkronizasyonu uygulanan genç kilis keçilerinde (çebiç) iki farklı zamanda eCG (500 IU) uygulamasının östrus ve gebelik oranları ile yavru sayıları üzerindeki etkileri araştırılmıştır. Daha önce hiç çiftleşmemiş olan 13-15 aylık 23 çebiçe, üreme sezonu dışında 11 gün süre ile progesteron emdirilmiş süngerler (20 mg flugestone acetate) ve sünger uzaklaştırılmasından 2 gün önce PGF2α (125 mcg cloprostenol) uygulandı. eCG; 1. gruptaki çebiçlere (n:11, -48. saat grubu) sünger uzaklaştırılmasından 2 gün önce uygulanırken, 2. gruptaki çebiçlere (n:12, 0. saat grubu) sünger uzaklaştırma gününde uygulandı. Tekelerin atlamasına izin veren çebiçler östrusta kabul edildi ve çiftleştirildi. Her iki gruptaki tüm çebiçler östrus gösterdi. Birinci (-48. saat) grubun gebelik (oğlaklama) oranı (sırasıyla %54,6 ve %25.0, P>0.05) ve toplam yavru sayısı (sırasıyla 7 ve 3, P>0.05) ikinci (0. saat) gruptan sayısal olarak daha yüksek bulundu, ancak gruplar arasındaki farklılıklar istatistiksel olarak önemsiz bulundu. Çalışmada; gebelik oranı ve toplam yavru sayısındaki sayısal artışın ekonomik değerleri göz önüne alındığında, Kilis çebiçlerinde üreme mevsimi dışında eCG'nin sünger uzaklaştırılma zamanından ziyade, sünger çıkarılmasından 48 saat önce uygulanmasının daha faydalı olabileceği sonucuna varılmıştır. Effects of eCG application time on fertility in off-season estrus synchronized Kilis goat kids ABSTRACTIn the study, the effects of eCG (500 IU) administration at two different times on estrus and pregnancy rates and the number of offspring were investigated in estrus-synchronized goat kids. Twenty three kilis goat kids 13-15 months old that had never mated before were treated out of the breeding season with a progesterone sponge (20 mg flugestone acetate) for 11 days and PGF2α (125 mcg cloprostenol) two days before sponge removal. While eCG was applied to kids in group 1 (n:11, hour -48 group) two days before sponge removal, it was applied to kids in group 2 (n:12, hour 0 group) on the day of sponge removal. Goat kids that allowed the bucks to jump were considered estrus and mated. All kids in both groups showed estrus. The pregnancy (kidding) rate (54.6% vs. 25.0%, respectively, P>0.05) and total number of offspring (7 vs. 3, respectively, P>0.05) of the 1. (hour -48) group were found numerically higher than the 2. (hour 0) group, but the differences between the groups were not statistically significant. Considering the economic values of numerical increase in the pregnancy rate and the total number of offspring, it was concluded that it may be more beneficial to apply eCG 48 hours before the sponge removal rather than at the time of sponge withdrawal out of the breeding season in Kilis goat kids.