Photosynthetic pigments of plants capture light as a source of energy for photosynthesis. However, the amount of energy absorbed often exceeds its utilization, thus causing damage to the photosynthetic apparatus. Plants possess several mechanisms to minimize such risks, including non-photochemical quenching (NPQ), which allows them to dissipate excess excitation energy in the form of harmless heat. However, under non-stressful conditions in indoor farming, it would be favorable to restrict the NPQ activity and increase plant photosynthetic performance by optimizing the light spectrum. Towards this goal, we investigated the dynamics of NPQ, photosynthetic properties, and antioxidant activity in the leaves of tomato plants grown under different light qualities: monochromatic red (R), green (G), or blue (B) light (L) at 80 µmol m−2 s−1 and R:G:B = 1:1:1 (referred to as the white light (WL)) at 120 µmol m−2 s−1. The results confirm that monochromatic BL increased the quantum efficiency of PSII and photosynthetic pigments accumulation. The RL and BL treatments enhanced the NPQ amplitude and showed negative effects on antioxidant enzyme activity. In contrast, plants grown solely under GL or WL presented a lower amplitude of NPQ due to the reduced accumulation of NPQ-related proteins, photosystem II (PSII) subunit S (PsbS), PROTON GRADIENT REGULATION-LIKE1 (PGRL1), cytochrome b6f subunit f (cytf) and violaxanthin de-epoxidase (VDE). Additionally, we noticed that plants grown under GL or RL presented an increased rate of lipid peroxidation. Overall, our results indicate the potential role of GL in lowering the NPQ amplitude, while the role of BL in the RGB spectrum is to ensure photosynthetic performance and photoprotective properties.

The objective of this study was to identify the effect of abscisic acid (ABA), putrescine (Put) and hydrogen peroxide (H2O2) foliar pre-treatment on drought tolerance of barley. Despite water limitation, ABA-sprayed plants preserved increased water content, photosynthetic efficiency of PSII (ΦPSII) and CO2 assimilation rate (Pn) compared to untreated stressed plants. The ABA-treated plants presented also the lowest rate of lipid peroxidation (MDA), lowered the rate of PSII primary acceptor reduction (1 – qP) and increased the yield of regulated energy dissipation (NPQ) with higher accumulation of PGRL1 (PROTON GRADIENT REGULATION LIKE1) protein. These plants preserved a similar level of photochemical efficiency and the rate of electron transport of PSII (ETRII) to the well-watered samples. The significantly less pronounced response was observed in Put-sprayed samples under drought. Additionally, the combined effects of drought and H2O2 application increased the 1 – qP and quantum yield of non-regulated energy dissipation in PSII (ΦNO) and reduced the accumulation of Rubisco activase (RCA). In conclusion, ABA foliar application allowed to balance water retention and preserve antioxidant capacity resulting in efficient photosynthesis and the restricted risk of oxidative damage under drought. Neither hydrogen peroxide nor putrescine has been able to ameliorate drought stress as effectively as ABA.

The artificial light used in growth chambers is usually devoid of green (G) light, which is considered to be less photosynthetically efficient than blue (B) or red (R) light. To verify the role of G light supplementation in the spectrum, we modified the RB spectrum by progressively replacing R light with an equal amount of G light. The tomato plants were cultivated under 100 µmol m–2 s–1 of five different combinations of R (35–75%) and G light (0–40%) in the presence of a fixed proportion of B light (25%) provided by light-emitting diodes (LEDs). Substituting G light for R altered the plant’s morphology and partitioning of biomass. We observed a decrease in the dry biomass of leaves, which was associated with increased biomass accumulation and the length of the roots. Moreover, plants previously grown under the RGB spectrum more efficiently utilized the B light that was applied to assess the effective quantum yield of photosystem II, as well as the G light when estimated with CO2 fixation using RB + G light-response curves. At the same time, the inclusion of G light in the growth spectrum reduced stomatal conductance (gs), transpiration (E) and altered stomatal traits, thus improving water-use efficiency. Besides this, the increasing contribution of G light in place of R light in the growth spectrum resulted in the progressive accumulation of phytochrome interacting factor 5, along with a lowered level of chalcone synthase and anthocyanins. However, the plants grown at 40% G light exhibited a decreased net photosynthetic rate (Pn), and consequently, a reduced dry biomass accumulation, accompanied by morphological and molecular traits related to shade-avoidance syndrome.

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