This study was carried out to determine the effects of dried grape pomace supplementation in a corn-soy-based diet on performance, egg quality, plasma and egg lipid peroxidation, and some biochemical parameters of laying hens. In this study, 96 moulted 80-week-old Bovans laying hens were distributed into three groups with eight replicates. The hens were fed a supplemented diet with 0% (control group), 4% and 6% grape pomace (experimental groups) for 12 weeks. In the study, the effects of grape pomace supplementation in a corn-soy-based diet on performance, internal and external egg quality, serum total cholesterol, total protein, glucose and triglyceride levels, and plasma and egg yolk malondialdehyde (MDA) levels were determined. The addition of grape pomace to laying hen diet did not significantly affect live weight, feed intake, egg production and feed efficiency (P > 0.05). In all treatment groups, supplementation did not significantly affect albumen index, Haugh unit, egg yolk index, yolk colour, eggshell ratio, eggshell thickness, egg-specific gravity, total cholesterol, total protein and triglyceride compared to the control group (P > 0.05). In the study, 4% grape pomace supplementation to diet significantly increased egg weight (P < 0.001), and both 4% and 6% grape pomace supplementation levels significantly increased liver weight (P < 0.05) and liver weight ratio (P < 0.01). Grape pomace addition to laying hens diet significantly decreased plasma MDA and serum glucose levels (P < 0.001). Also, grape pomace addition significantly decreased egg yolk MDA levels on day 1 (incubation on 0 minute; P < 0.001), and day 15 [incubation on 0, 30, 60 and 90 (P < 0.05) minutes]. The results of this study concluded that performance, egg quality and serum total cholesterol, total protein and triglyceride levels were not negatively affected. Plasma and egg yolk MDA, and serum glucose levels were reduced by 4% and 6% supplementation to laying hen diets. It was concluded that grape pomace supplementation has the potential to extend shelf life.

In this study, the effects of replacing inorganic copper, zinc and manganese with different levels of organic complexes of the same trace minerals on the lipid peroxidation and antioxidant defense systems in broilers were investigated. Two-hundred Ross-308 one-day-old broiler chickens were placed on controlled diets until 42 d of age. The experimental animals were divided into four groups comprising three experimental groups and one control group, each consisting of 50 chickens. All groups were also divided into five subgroups each containing 10 broiler chicks. The mineral content of the control group diet was controlled using a standard inorganic mineral premix with supplement levels and sources of trace minerals typical of commercial broiler diets according to the National Research Council (NRC) (containing 8 mg Cu as CuSO 4 , 40 mg Zn as ZnSO 4 , and 60 mg Mn as MnO, per kg). In the experimental diets, mineral premix was also comprised of inorganic formulations, except for those of Cu, Zn and Mn. Organically-complexed Cu, Zn, and Mn were separately added to the basal diet at 1/3 (L1), 2/3 (L2) and 3/3 (L3) levels with respect to the NRC recommendation, as Bioplex Cu TM , Bioplex Zn TM , Bioplex Mn TM . At the end of the trial, the plasma Zn level significantly increased when the plasma Cu level significantly decreased (p<0.05) in chickens fed at 2/3 and 3/3 levels of organically complexed minerals. The liver trace mineral concentrations were significantly higher in chickens fed inorganic trace minerals in comparison to those fed organically-complexed minerals. The plasma malondialdehyde (MDA) level of experimental chickens was decreased in groups receiving levels of organic Cu, Zn and Mn in comparison to those fed inorganic forms (p<0.01). The erythrocyte superoxide dismutase (SOD) activity was higher in all groups receiving the organic mineral supplements in comparison to those fed inorganic forms (p<0.01). No differences were observed on either the erythrocyte catalase (CAT) activity or the plasma ceruloplasmin (Cp) levels, and the liver MDA levels and liver CAT and SOD activities in any of the groups that received the organic supplements of Cu, Zn, and Mn. It was concluded that supplementation of lower levels of organically-complexed copper, zinc, and manganese instead of their inorganic forms in diets had no negative effects on the antioxidant defense system in broilers.

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