Catheter-associated urinary tract infections (CAUTIs) is the most common type of hospital acquired infection in Intensive Care Units. It is associated with significant morbidity, length of hospital stay and cost of hospitalization. Preventing CAUTI is essential and critical care nnurses are the key to accomplishing this goal. Aim: -evaluate the efficacy of protocol of hygienic care by chlorhexidine gluconate on the occurrence of a catheter associated urinary tract infection among critically ill patients. Desgin and setting: -A quasi-experimental study was conducted at the Neurological Intensive Care Unit of both Tanta University Hospital and Neuro-psychiatry center at Tanta Main University Hospital. A convenience sampling of 60 adult patients with indwelling urinary catheters were selected and divided into two equal groups 30 patients in each group. Group 1:-(Control group), they received routine nursing care by hospital nursing staff. Group 2:-(Study group), they received application of protocol of hygienic care by cholorohexidine gluconate 2%. Three tools were used for data collection .Tool (I) Assessment tool which included Patient's socio-demographic characteristics. Tool (II)): Catheter Associated Urinary Tract Infection Assessment Profile (CAP):-. Tool (III)): Infection Assessment Sheet: -. Results: -The main results revealed that the incidence rate of CAUTI among control group subjects was 93.33% while among study group subjects was 40.00%.Conclusions and recommendations: -Application of protocol of daily Hygienic Care by Chlorhexidine Gluconate has a significance effectiveness on reducing CAUTI among critically ill patients .It was recommended that all critically -ill patients with indwelling urinary catheter in ICU should receive daily Hygienic Care by Chlorhexidine Gluconate as routine of care.