Wine grape (Vitis vinifera L.) is the most widely cultivated fruit crop in the world. However, the climactic characteristics in some growing regions are suboptimal for grape production, including short season length and excess precipitation. Grape growers can utilize an array of methods to mitigate these issues, including “early leaf removal,” a management practice involving the removal of leaves from selected basal nodes along shoots around bloom. This meta-analysis reviews the extensive literature on this practice, with specific regards to application at “pre-bloom” (PB). One hundred seventy-five publications on the topic of “early leaf removal” were identified using key terms and subsequently narrowed via eight data curation steps. The comparison between treated (PB) and control plants in these studies revealed two important results. First, PB lowered bunch rot disease (−61%), partially through reducing the compactness of clusters. Second, PB promoted a significant increase in fruit total soluble solids (°Brix, +5.2%), which was related to the increase in the leaf-to-fruit ratio. Furthermore, cultivar and rootstock were found to have a large influence on the success of PB, while the contribution of climate was smaller. In conclusion, PB significantly lowers yield and bunch rot disease and increases °Brix, both of which improve grape and wine quality.

In cool-climate viticulture, the short growing season can influence grape seed maturation by reducing the apparent oxidation of flavan-3-ol monomers and associated increase in seed browning. A reduction in seed maturation increases the potential extraction of flavan-3-ol monomers into wine during maceration operations, heightening bitterness. Here, we carried out a 2 × 2 factorial experiment to test the ability of freezing and heating treatments to advance maturation (decrease flavan-3-ol, improve browning) of (Vitis vinifera L.) Pinot noir and Cabernet Sauvignon seeds over a 24-h incubation period. Only freezing significantly increased seed browning in both cultivars. Subsequent correlations with seed flavan-3-ol monomer concentrations suggest that freezing enhanced the oxidation of these compounds. Interestingly, natural ripening and freezing reduced galloylated flavan-3-ol monomers to a greater extent than non-galloylated ones. This study provides new information regarding the susceptibility of flavan-3-ol monomers to freezing and heating, and also suggests that freezing can advance the maturation the seeds of under-ripe red vinifera grapes.

Beside prevalent methods for determination of genetic purity, image analysis technique can also be used with a high reliability. For evaluation of image analysis application on determination of genetic purity of wheat varieties, a research was carried out at seed and plant certification and registration research institute (SPCRI) in Karaj, Iran, 2011-12. So, BITA-technique (Biochemical and Imaging technique for Analysis) and BITA-image analyzer were used for assessment of genetic purity. Based on the results, there was no significant difference between formal and informal seed production in Tehran province while meaningful difference was observed between two seed production system in Kermanshah and west Azerbaijan provinces in a way that higher seed genetic purity was observed in formal section. Generally, the maximum genetic purity (99%) was observed in both formal and informal wheat seed production systems in Mahidasht and Naghadeh areas of Kermanshah and West Azerbaijan Provinces, while the lowest genetic purity (26%) was related to formal seed production system in Ravansar area of Kermanshah Province. So, for producing seeds with higher genetic purity, the current managements and inspections in seed production systems should be evaluated for identifying the problems and planning for solving them.

Red Vitis vinifera cultivars are often limited in their performance by climate conditions (e.g., rain, humidity, cold winters, and cool summers). Cool climate viticulture regions are characterized by short growing seasons that reduce fruit quality, limiting technological and phenolic maturity. Management of fruit technological ripening, in vineyard or post-harvest, is pivotal for wine quality. However, the impact of vineyard or cellar practices on seed phenolic fraction remains poorly understood. The aim of our project was to evaluate seed color change, phenolic composition, and their extraction potential after an oxidation induced by a freezing treatment. The freezing treatment was followed by 24 hours of incubation at different temperatures in two Vitis vinifera cultivars: Pinot noir and Cabernet Sauvignon. Results are reporting that the freezing caused the seed color darkening and significant phenolic changes, suggesting similarities with the natural process. The phenolic evolution reported different behaviour between cultivars and compound classes. Most of the changes occurred during the first three hours of incubation, indicating that the oxidation reactions take place at the beginning of the thawing process.

In cool-climate viticulture, the short growing season can influence grape seed maturation by reducing the apparent oxidation of flavan-3-ols and associated increase in seed browning. A reduction in seed maturation increases the potential extraction of flavan-3-ols into wine during maceration operations, heightening bitterness. Here, we carried out a 2x2 factorial experiment to test the ability of freezing and heating treatments to artificially “ripen” seeds (decrease flavan-3-ols, improve browning) of (Vitis vinifera L.) Pinot noir and Cabernet Sauvignon over a 24-hour incubation period. Only freezing significantly increased seed browning in both cultivars. Subsequent correlations with seed flavan-3-ols concentrations suggest that freezing enhanced the oxidation of these compounds. Interestingly, natural ripening and freezing reduced galloylated flavan-3-ols to a greater extent than non-galloylated ones. This study provides new information regarding the susceptibility of flavan-3-ols to freezing and heating, and also suggests that freezing can artificially ripen the seeds of under-ripe red vinifera grapes.