Pasture-fed cattle yield carcasses with yellow fat; consumers often reject the resulting meat products because they assume they come from old and/or culled animals. Recombinant bacteria expressing beta-carotene 15, 15'-monooxygenase, introduced into the rumen of the animal, might help to reduce the coloration since this enzyme converts carotene to retinal, thereby eliminating the source of yellowness. The goal of this work was to evaluate the effect of a recombinant beta-carotene 15, 15'-monooxygenase (BCMO1) from Gallus gallus, expressed in Escherichia coli. The genetically modified microbe was introduced into ruminal fluid, and carotene conversion to retinal was measured. Under optimum conditions the enzyme produced 6.8 nmol of retinal per 1 mg of protein in 1 hour at 37 °C. The data on in vitro digestibility in ruminal fluid showed no differences in beta-carotene breakdown or in retinal production (p > 0.1) between E. coli with pBAD vector alone and E. coli with pBAD/BCMO1. The pBAD/BCMO1 plasmid was stable in E. coli for 750 generations. These results indicate that the protein did not break beta-carotene into retinal in ruminal fluid, perhaps due to its location in the periplasmic space in E. coli. Future research must consider strategies to release the enzyme into the rumen environment.

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Meat is an excellent medium for bacterial growth due to its high water and nutrient content. The nitrogenous compounds (NC) are derived through decarboxylation of amino acids due to microbial enzymes. The objective of this study was to evaluate the concentration of 3 NC and the proliferation of some microorganisms in rabbit meat with three treatments (T) , classified by three types of packaging for 21 days (d) in rabbit meat stored cold. The meat samples were obtained of the Longissimus thoracis et lumborum muscle. Each sample was divided and two groups were formed. The first group was used to measure the physicochemical characteristics of the meat, and the second group was used to quantify NC and bacterial isolation. The pH in the meat decreased from 0 to 21 d in the three T. The brightness (L*) decreased (P<0.05), while the variables a* and b* increased (P<0.05) to 21 d for all groups. Histamine and cadaverine remained low and were similar in the three T (P>0.05). Putrescine (PU) increased (P<0.05) from 7 to 21 d in the Control-Plastic (CP) and Semi-permeable plastic film (SP) groups vs. Vacuum packing (VP). The Enterobacteriaceae remained constant throughout the experimental period in the three T, compared to the aerobic mesophilic, which was higher (P<0.05) until 21 d of the evaluation in CP and SP. The type of packaging and cooling time influenced the concentration of NC. The VP had the lowest level of PU and mesophilic bacteria until 21 d of storage.