Studies have proposed that Porphyromonas gingivalis (Pg) and Tannerella forsythia (Tf) promote a nonspecific inflammatory response that could produce systemic disease. Oral inoculation of Pg and Tf on the immune and arthritis response was evaluated in BALB/C mice divided into four groups: (1) sham; (2) food contaminated with Pg/Tf; (3) complete Freund's adjuvant (CFA) + Pg/Tf; and (4) CFA alone. CFA was administered subcutaneously on days 1 and 14. The arthritis response was monitored for 21 days after day 14 of CFA administration. IL-1β and IL-6 were determined in serum. T cell activation was evaluated by CD25 in salivary lymph nodes or mouse spleen. Pad inflammation appeared by day 19 in the CFA group, but animals with bacteria inoculation presented a delay. A significant increase in IL-6 was found in Groups 3 and 4, but not with respect to IL-1β. We observed an increase in CD25 in cells derived from cervical nodes and in animals with bacteria inoculation and CFA. A local immune response was observed in mice inoculated with Pg and Tf (T cell activation); a systemic response was observed with CFA. Since pad inflammation was delayed by bacterial inoculation this suggests that local T cell activation could decrease pad inflammation.
<div class="page" title="Page 1"><div class="layoutArea"><div class="column"><p><span>Introducción: </span><span>La pulpa dental humana es considerada una fuente </span><span>celular, de la que se puede obtener fibroblastos, odontoblastos y cé</span><span>- </span><span>lulas troncales. Actualmente, cultivos de estas células son usados </span><span>en estudios de biocompatibilidad de materiales dentales, así como en medicina regenerativa e ingeniería tisular. El tiempo postexodon- cia conforma un factor importante para la obtención de un elevado porcentaje de viabilidad celular. Reportes sobre la viabilidad celular </span><span>de cultivos de células troncales de la pulpa dental no incluyen la po</span><span>- blación celular total de la pulpa. Por este motivo, el objetivo de este </span><span>estudio fue conocer la influencia del tiempo postexodoncia, la edad y el sexo del paciente en la obtención de células viables de pulpas den</span><span>- tales. </span><span>Material y métodos: </span><span>Se compararon dos tiempos, antes de las </span><span>24 horas y después de las 72 horas postexodoncia. Se recolectaron </span><span>un total de 42 dientes de pacientes entre 13 y 42 años de edad. Para determinar la viabilidad celular se usó la tinción de azul de tripano y </span><span>se hizo el recuento celular en el microscopio. Las células vivas fueron</span><span>observadas de color blanco y las muertas de color azul. </span><span>Resultados: </span><span>Las pulpas que fueron obtenidas antes de las 24 horas postexodoncia presentaron un mayor porcentaje de viabilidad celular con respecto a </span><span>las pulpas obtenidas después de las 72 horas postexodoncia. </span><span>Con- clusiones: </span><span>La obtención de las células de la pulpa dental debe reali</span><span>- zarse en un tiempo </span><span>≤ </span><span>a 24 horas postexodoncia para óptimos resulta- dos. Existe una relación entre la edad del paciente y la viabilidad en las pulpas obtenidas antes de las 24 horas postexodoncia. El sexo del </span><span>paciente no influye en la viabilidad celular de los tiempos estudiados.</span></p></div></div></div>
Determine the efficacy of 5 % chlorine dioxide as an endodontic irrigant for pulp dissolution. Thirty five samples of human dental pulp were obtained, previously weighed and immersed in three solutions= 5 % ClO 2 , 5.25 % NaOCl and saline (control group), for 10 minutes at 32 ºC; they were dried and reweighed. Then the weight loss was compared to the original weight and analyzed statistically. 5.25 % NaOCl and 5 % ClO 2 dissolved the dental pulp samples more effectively than saline (p> 0.001). No statistically significant difference was found between the tissue dissolving properties of 5.25 % NaOCl and 5 % ClO 2 (p=0.893). 5 % ClO 2 is effective in dissolving human dental pulp tissue.
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