Objective:The study was conducted to determine the relationship between health-promoting lifestyle behaviors and sleep quality of nurses working in the pediatrics clinics. Methods:The study was conducted among nurses working in the pediatrics clinic of a training and research hospital located in Istanbul. Nurse Information Form, Health-Promoting Lifestyle Profile-II (HPLP-II), and Pittsburg Sleep Quality Index (PSQI) were applied to the nurses. Ethics committee and institutional permission, permission from the scale authors via e-mail, and written consent from the nurses were obtained in the study.Besides the descriptive statistics, Mann Whitney U test, Kruskal Wallis test, and Spearman's Rho Correlation analysis were used for the analysis of the study.Results: Among the 200 nurses that were surveyed, 121 (60.5%) responded.81% of the nurses (n=98) were female, and 80.2% had a bachelor's degree. Age average of the nurses was 25.74±3.86 years and the average weekly working hours was 49.40±7.70 hours. It was determined that total mean score of HPLP-II was 125.26±16.40 and PSQI total mean score was 12.13±2.29. The lowest mean HPLP-II subscale score was 16.19±4.64 for exercise and 17.71±3.74 for stress management. A statistically significant correlation was found between the HPLP-II total scores and PSQI total scores (r= -0.19; p< 0.05). Conclusion:It was determined that the health-promoting lifestyle behaviors of the nurses were above the moderate level, their sleep quality was poor, and there was a significant correlation between the health-promoting lifestyle behaviors and the sleep quality of the nurses. It can be suggested by nursing to conduct the studies determining the practices that will improve the health-promoting lifestyle behaviors of the nurses and enhance their sleep quality.