Purpose:The purpose of this in vitro study was to compare the post space volume changes following removal of glass fiber posts in endodontically treated teeth by using erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser to the conventional ultrasonic method. Materials and Methods: Twelve single-root human extracted teeth were cut into 13 mm near cementoenamel junction (CEJ), and then underwent endodontic treatment. The post spaces were prepared to 8 mm in length. Glass fiber posts were inserted with self-curing resin cement. Specimens were randomly assigned to laser or ultrasonic methods for their post removal. Post space volumes were measured using microcomputed tomography (micro-CT) before post cementation and after post removal. Dentin thickness was measured after post removal at coronal, middle, and apical third of the root canal space. Paired t-test and t-test were used to compare space volumes between before post cementation and after post removal, and between laser and ultrasonic groups, respectively. Results: Six specimens were tested for each group. The average volume change was 6.499 mm 3 in laser and 7.418 mm 3 in ultrasonic method. There was not a significant difference between laser and ultrasonic group in respect of post space volume changes (p = 0.71). Both methods showed significant volume increase following post removal (p < 0.05). Significantly less dentin was lost when laser was used for post removal in the coronal portion of the post space (p = 0.002). Conclusions: Er,Cr:YSGG laser can be used as effective option, comparable to the conventional ultrasonic method when removing posts in endodontically treated teeth. Laser has the potential to provide conservative post removal.