Background: Pelvic organs prolapse is considered as one of the most common cause of reproductive health problem which influences the women ' s quality of life. This study aimed to assess knowledge of primiparous women about genital prolapse & implement educational program to these women about postpartum preventive measures of genital prolapse at Woman's Health Hospital. Patients and methods: This is a quasi-experimental research design; the study was conducted at the postpartum ward and reception unit at Women's Health Hospital, Assiut University. The simple random sample included 500 Primiparous women; the data were collected through three tools: a structured interview questionnaire, pretest &posttest and booklet. Results: That there was a statistically significant difference between pretest knowledge level and posttest knowledge level about genital prolapse with p-value =0.000, the present study showed majority of women had lack of knowledge regarding preventive measures of genital prolapse Conclusions: The findings of the study showed a significant improvement in knowledge regarding preventive measures of genital prolapse. Recommendations: Implementation of educational program for maternity nurses to increases awareness about risk factors and preventive measures of genital prolapse.