Aim:Broilers’ optimum performance in response to their genetic potential depends on litter environment which is ideal for bacterial survival and coccidian oocyst sporulation. An in vitro evaluation was conducted for the effectiveness of superphosphate, meta-bisulfide, and charcoal litter amendments in minimizing Escherichia coli O157:H7 and Salmonella Typhimurium survival, Eimeria oocyst count, and sporulation.Materials and Methods:Three groups of 16 litter trays were prepared and inoculated with E. coli O157:H7, S. Typhimurium, and Eimeria non-sporulated oocyst. A set of four trays in each group was designed for each one of the chemical amendments. A total of 720 litter samples were collected and examined for bacterial counts, Eimeria oocyst count, and sporulation during the experimental period (35 days).Results:Litter moisture and pH revealed a highly significant (p<0.001) reduction in all treated litter trays compared to control. Total bacterial count (TBC), total Enterobacteriaceae count, and S. Typhimurium count showed a highly significant (p<0.001) reduction in meta-bisulfide-treated trays compared to other amendments and positive control. Meanwhile, Eimeria oocyst count and sporulation revealed a highly significant (p<0.001) reduction in superphosphate, meta-bisulfide, and charcoal-treated trays, respectively. Temperature revealed a highly significant (p<0.001) weak positive correlation with pH of all inoculated trays, a highly significant (p<0.001) weak negative correlation with moisture percentage of E. coli O157:H7 and S. Typhimurium inoculated trays, and a highly significant (p<0.001) weak negative correlation with TBC. Meanwhile, relative humidity revealed significant (p≤0.005) weak positive correlation with moisture percentage of E. coli O157:H7 inoculated trays.Conclusion:The study concluded that regular usage with periodical reapplication of litter amendments as meta-bisulfide or superphosphate in poultry farms is one of the indispensable managemental and preventive measures for minimizing bacterial survival and inhibiting Eimeria oocyst maturation and sporulation.

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Background and Aim:Prophylaxis and disease prevention is an essential strategy among biorisk management in poultry farms that stimulate and maintain the birds’ immunity. The aim of this study was to investigate the prophylactic, and immune-stimulant influence of Nigella sativa Linn. in broilers under biological stress.Materials and Methods:A total of 250 1-day-old (ross) chicks were divided into 5 groups; four of which were supplemented with 1.4%, 2.8%, 4.2%, and 5.6% N. sativa Linn., respectively. The four supplemented groups were challenged with Escherichia coli O157:H7 1.5×108 at a 14th day old. A total of 1050 samples (150 serum, 150 swab, and 750 organ samples) were collected and examined.Results:A highly significant increase (p<0.01) in 5.6% N. sativa Linn. supplemented group in performance traits (body weight, weight gain, and performance index), biochemical parameters (proteinogram, liver enzymes, and creatinine), immunoglobulins concentration, and immune organs’ weight. Meanwile, liver showed improvement of histoarchitecture without fibrosis. Heart showed a mild pericarditis with a mild degree of hydropic degeneration. Bursa, thymus, and spleen showed lymphoid hyperplasia.Conclusion:A concentration of 5.6% N. sativa Linn. in broiler’s feed can improve the immune response and subsequent resistance of broilers against diseases.

Background and Aim:Birds litter contains unutilized nitrogen in the form of uric acid that is converted into ammonia; a fact that does not only affect poultry performance but also has a negative effect on people’s health around the farm and contributes in the environmental degradation. The influence of microclimatic ammonia emissions on Ross and Hubbard broilers reared in different housing systems at two consecutive seasons (fall and winter) was evaluated using a discriminant function analysis to differentiate between Ross and Hubbard breeds.Materials and Methods:A total number of 400 air samples were collected and analyzed for ammonia levels during the experimental period. Data were analyzed using univariate and multivariate statistical methods.Results:Ammonia levels were significantly higher (p< 0.01) in the Ross compared to the Hubbard breed farm, although no significant differences (p>0.05) were found between the two farms in body weight, body weight gain, feed intake, feed conversion ratio, and performance index (PI) of broilers. Body weight; weight gain and PI had increased values (p< 0.01) during fall compared to winter irrespective of broiler breed. Ammonia emissions were positively (although weekly) correlated with the ambient relative humidity (r=0.383; p< 0.01), but not with the ambient temperature (r=−0.045; p>0.05). Test of significance of discriminant function analysis did not show a classification based on the studied traits suggesting that they cannot been used as predictor variables. The percentage of correct classification was 52% and it was improved after deletion of highly correlated traits to 57%.Conclusion:The study revealed that broiler’s growth was negatively affected by increased microclimatic ammonia concentrations and recommended the analysis of broilers’ growth performance parameters data using multivariate discriminant function analysis.

Background and Aim: Manipulating lighting colors and regimens is considered an effective mean for improving broiler productivity. The influence of red, blue, and white light-emitting diode (LED) was investigated using three different regimens of lighting and darkness; continuous 23 h light (L):1 h dark (D), continuous 18 h L:6 h D, and intermittent 16 h L:8 h D hours on the performance, carcass weight (CW), feed and water intake (WI), serum glucose (GLUCO), triglycerides (TG), and cholesterol (TC), intestinal bacterial load, growth and metabolic hormones, and efficiency of Newcastle disease (ND) vaccine. Materials and Methods: A total of 252 1-day-old Ross broilers on deep litter were divided into nine groups. The 1st, 4th, and 7th groups were exposed to continuous 23L:1D, the 2nd, 5th, and 8th groups were exposed to continuous 18L:6D, and the 3rd, 6th, and 9th groups were exposed to intermittent 16L:8D (4L:2D, 4 times) lighting regimen using red, blue, and white LED lights, respectively. A total of 1350 samples (225 sera, 225 swabs, and 900 organ samples) were collected. Results: Blue LED group revealed a highly significant increase (p<0.01) in live body weight, body weight gain, performance index, CW, spleen, heart, and liver weights, and anti-ND antibody titer, as well as a highly significant decline (p<0.01) of feed intake, WI, GLUCO, TG, TC, growth hormone, insulin, tri-iodothyronine (T3), tetra-iodothyronine (T4), total bacterial count (TBC), and total Enterobacteriaceae count compared to red and white LED lights in all tested lighting regimens. Continuous 23L:1D and 18L:6D regimens were significantly (p<0.01) superior to intermittent 16L:8D in their influence on the performance, CW, biochemistry, hormonal profile, and bacterial load. Conclusion: The blue LED light associated with continuous 18L:6D or 23L:1D h regimen is highly recommended in broiler houses for their enhancing the productive performance, growth, and immunity.