In Burkina Faso, deficiency of amino acids in protein is becoming a major healthy public problem. This study was purposed to optimize essentials amino acids in single cell protein (SCP) by supplementing different nitrogen sources during fermentation of mango waste with Candida utilis FJM12. Analytical methods were used to determine biomass yield, chemical composition and amino acids profile of SCP. The principal component analysis (PCA) method was performed to identify the nitrogen source which exhibited best rate of SCP. The maximum biomass yield (6.48±0.03 g/L) exhibited 9.65±0.36% (w/w) of ash, while using yeast extract. The proximate composition of SCP revealed 56. 40±1.30, 13.25±0.11, 3.80±0.10 and 6.60±0.25% (w/w), respectively for crude protein, lipids, carbohydrates, and nucleic acid content. PCA showed a strong correlation between yeast extract and ammonium sulphate and demonstrated their positive influence to increase the rate of SCP and essentials amino acids as compared to Food and Agriculture Organization (FAO) recommendation. These results demonstrated that C. utilis FJM12 could be suitable for essentials amino acids.

The urinary tract infection (UTI) is the most common bacterial infection, especially in women. The increased incidence of UTIs, at the last decades have paralleled with the growing emergence of antibiotic resistance. The aim is to evaluate aqueous garlic extract (AGE) susceptibility against multidrug-resistant (MDR) bacteria isolated in urine of women. The investigation of antibacterial propriety and time kill effect of AGE was performed by the well method, microdilution method and spectrophotometer assay. Antibiotics susceptibility assay revealed that the nine MDR bacteria had high resistance against Amoxicillin/ clavulanic acid (100%) and Erythromycin (100%), Cefotaxime (83.33%) and Ceftazidime (83.33%). AGE exhibited potent antibacterial activity against the nine MDR bacteria tested. In Gram-negative bacteria, the inhibition diameters ranged from 20 ± 3 to 32 ± 4 mm, with Minimum Inhibitory Concentrations (MICs) ranging from 10% to 12.5% (w/v) and Minimum Bactericidal Concentration (MBCs) was 12.5 % (w/v). Gram-positive bacteria exhibited diameters ranging from 38 ± 2 to 45 ± 1 mm; MIC and MBC values ranged from 05 to 10 % (w/v) and were found more susceptible than Gram-negative bacteria. To conclude, this investigation shown that AGE have high potential antibacterial to use as an alternative to treat women UTIs.

Urinary tract infections are the first infections in pregnant women and can cause serious complications during pregnancy. In order to improve its management in low income country like Burkina Faso, we conducted a prospect cross-sectional study, to describe its epidemiological and biological aspects in pregnant women at Souro Sanou hospital at Bobo-Dioulasso (Burkina Faso).

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Les auteurs remercient le programme PACER de l'UEMOA pour avoir soutenu cette étude. RESUME Les bactéries à Gram positif et à Gram négatif et les archées produisent respectivement des bactériocines et des archéocines. Les bactériocines et les archéocines sont des peptides antimicrobiens. Au fil des ans leur rôle dans la sûreté des produits alimentaires n'a cessé d'être démontré, faisant de ces peptides naturels un centre d'intérêt pour la recherche scientifique. Cette revue tente de faire l'état des connaissances sur les bactériocines produites par les bactéries Gram positif, bactéries Gram négatif et les archées en faisant ressortir leurs organisations génétiques particulières, leurs modes d'action qui diffèrent de celui des antibiotiques et surtout leurs larges spectres d'action. La collecte des données a consisté à consulter les articles scientifiques publiés et les mémoires de thèses sur les différents travaux menés sur les bactériocines et disponible en ligne via les journaux de publication et les sites dédiés aux collectes de thèses en ligne. Ces peptides antimicrobiens pourraient constituer une alternative pour la conservation des denrées alimentaires surtout en milieu rural dans les pays en voie de développement.