“Biogeophysics” is a rapidly evolving Earth science discipline concerned with the geophysical signatures of microbial interactions with geologic media. It spans the established disciplines of geomicrobiology, biogeoscience, and geophysics. Biogeophysics research in the last decade has confirmed the potential for geophysical techniques to measure not simply the physical and chemical properties of the subsurface, as already well established, but also to detect microbes, microbial growth, and microbe‐mineral interactions, thus representing a major paradigm shift in geophysical thinking. In this review we begin by defining biogeophysics and provide a historical perspective. We then consider microbial alterations of petrophysical properties as such alteration is the source of most biogeophysical signals. Our review then focuses on geophysical interrogation of microbial processes, including the direct detection of microbial cells and biofilm formation, microbial metabolic by‐products, microbe‐mediated redox processes, and biogeochemical and microbe‐mineral transformations. We conclude by discussing challenges, opportunities, and potential new applications of biogeophysics to the exploration of life in extreme environments, e.g., the deep biosphere, the cryosphere, and other planets. We find that published biogeophysics studies to date are mostly observation based, presenting only empirical relationships between microbial and geophysical variables. Future research endeavors must focus on developing theoretical and/or numerical models for predicting geophysical signals arising from microbial activity.

We integrated temporal aeromagnetic data and recent earthquake data to address the long‐standing question on the role of preexisting Precambrian structures in modulating strain accommodation and subsequent ruptures leading to seismic events within the East African Rift System. We used aeromagnetic data to elucidate the relationship between the locations of the 2009 Mw 6.0 Karonga, Malawi, earthquake surface ruptures and buried basement faults along the hinge zone of the half‐graben comprising the North Basin of the Malawi Rift. Through the application of derivative filters and depth‐to‐magnetic‐source modeling, we identified and constrained the trend of the Precambrian metamorphic fabrics and correlated them to the three‐dimensional structure of buried basement faults. Our results reveal an unprecedented detail of the basement fabric dominated by high‐frequency WNW to NW trending magnetic lineaments associated with the Precambrian Mughese Shear Zone fabric. The high‐frequency magnetic lineaments are superimposed by lower frequency NNW trending magnetic lineaments associated with possible Cenozoic faults. Surface ruptures associated with the 2009 Mw 6.0 Karonga earthquake swarm aligned with one of the NNW‐trending magnetic lineaments defining a normal fault that is characterized by right‐stepping segments along its northern half and coalesced segments on its southern half. Fault geometries, regional kinematics, and spatial distribution of seismicity suggest that seismogenic faults reactivated the basement fabric found along the half‐graben hinge zone. We suggest that focusing of strain accommodation and seismicity along the half‐graben hinge zone is facilitated and modulated by the presence of the basement fabric.

Complex conductivity measurements (0.1–1000 Hz) were obtained from biostimulated sand‐packed columns to investigate the effect of microbial growth and biofilm formation on the electrical properties of porous media. Microbial growth was verified by direct microbial counts, pH measurements, and environmental scanning electron microscope imaging. Peaks in imaginary (interfacial) conductivity in the biostimulated columns were coincident with peaks in the microbial cell concentrations extracted from sands. However, the real conductivity component showed no discernible relationship to microbial cell concentration. We suggest that the observed dynamic changes in the imaginary conductivity (σ″) arise from the growth and attachment of microbial cells and biofilms to sand surfaces. We conclude that complex conductivity techniques, specifically imaginary conductivity measurements are a proxy indicator for microbial growth and biofilm formation in porous media. Our results have implications for microbial enhanced oil recovery, CO2 sequestration, bioremediation, and astrobiology studies.

[1] Although recent research suggests that contaminant plumes behave as geobatteries that produce an electrical current in the ground, no associated model exists that honors both geophysical and biogeochemical constraints. Here, we develop such a model to explain the two main electrochemical contributions to self-potential signals in contaminated areas. Both contributions are associated with the gradient of the activity of two types of charge carriers, ions and electrons. In the case of electrons, bacteria act as catalysts for reducing the activation energy needed to exchange the electrons between electron donors and electron acceptors. Possible mechanisms that facilitate electron migration include iron oxides, clays, and conductive biological materials, such as bacterial conductive pili or other conductive extracellular polymeric substances. Because we explicitly consider the role of biotic processes in the geobattery model, we coined the term ''biogeobattery.'' After theoretical development of the biogeobattery model, we compare model predictions with self-potential responses associated with laboratory and field scale investigations conducted in contaminated environments. We demonstrate that the amplitude and polarity of large (>100 mV) self-potential signatures requires the presence of an electronic conductor to serve as a bridge between electron donors and acceptors. Small self-potential anomalies imply that electron donors and electron acceptors are not directly interconnected, but instead result simply from the gradient of the activity of the ionic species that are present in the system.