Grain yield in wheat is the resultant of several plant attributes. It is very important to assess heritable variation involved in the inheritance of these attributes in addition to find the best combining genotypes. For this purpose, the present study involving 5 × 5 full diallel analysis was performed. Twenty F 1 hybrids along with their parents (9797, 9801, 9802, were planted in field using randomized complete block design (RCBD) with three replications in the research area of Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad during 2015-2016. Plant characters like plant height, flag leaf area, spike length, No. of fertile tillers per plant, No. of grains per spike, No. of spikelets per spike, 1000 grain weight and grain yield per plant were studied in this experiment. Mean squares due to general combining ability (GCA) were highly significant for all the traits except for spike length for which GCA effects were significant. Mean squares due to specific combining ability (SCA) and reciprocal combining ability (RCA) were highly significant for all the characters studied. GCA variance was higher than the SCA variance for spike length and No. of grains per spike exhibiting the predominant role of additive genetic variation in the inheritance of these traits. However, for the characters like plant height, flag leaf area, No. of fertile tillers per plant, No. of spikelets per spike, 1000 grain weight and grain yield per plant, the value of SCA variance was higher than the value of GCA variance showing non additive gene action for these How to cite this paper: Parveen, N., Kanwal, A., Amin, E., Shahzadi, F., Aleem, S., Tahir