This study evaluated, at a laboratory scale, samples composed of wastewater treatment sludge, blood, and swine manure, which were subjected to the process of anaerobic co-digestion. The goal of the study is to identify the reduction of organic loads through the removal of available carbon and its conversion to biogas. The results showed variation in hydraulic retention time, and that the total average volumes of biogas generated in 1-liter (L) reactors filled with 600 milliliters (ml) of samples, ranged from 3.37 to 18.76 L, with percentages of methane ranging between 57.9% and 71.1%. The largest volumes of biogas resulted from blends with lower percentages of swine manure, where the initial amounts of carbon in milligrams per liter (mg/L) were larger within the samples. All of the blends submitted to anaerobic treatment showed reductions in carbon content, and consequently, in chemical and biochemical oxygen demand, demonstrating that the efficiency of biodigesters in the treatment of waste is variable and related to physical-chemical factors, such as the amount of carbon available. K E Y W O R D Smethane, organic matter, renewable energy

The present study evaluated the potential for biogas generation from microalgae (MA) biomass and macrophytes used in vertical flow constructed wetlands (VFCW). The samples were obtained by separation and collection of MA after a hydraulic retention time of 14 days, frozen and taken to the laboratory, while the macrophytes of VFCW were obtained, by pruning, every 6 months. The obtained results presented reductions of 63.22% and 61.18% for COD and BOD 5 , respectively, and removal efficiencies of 53.91% for TP and 99.98% de N-NH 3 . Average biogas generation was 2322.51 NmL-gSV −1 with 54.61% CH 4 (winter/2019), 4491.47 Nml-gSV −1 with 57.17% CH 4 (spring/2019), 680.78 NmL-gSV −1 with 16.04% CH 4 (summer/2020), and 681.0 NmL-gSV −1 with 19.86% CH 4 (autumn/2020) for MA biomass and generation of biogas of 3826.70 NmL-gSV −1 with 44.26% CH 4 for VFCW biomass in winter and spring/2019 and of 829.68 NmL-gSV −1 with 17.06% CH 4 in summer and autumn/2020. Regarding electricity generation, the present work obtained 1.50 kWh/m 3 , therefore reaching similar values to other studies that used more traditional biomass sources.