The portable lysis apparatus provides a standalone, rapid, low cost and power efficient way of obtaining genomic constituents prior to a variety of bioassays used in the field of environmental, biomedical and other applied microbiology.

Kenalog is a synthetic glucocorticoid drug used to treat various cancers including ocular and choroidal melanoma. However, the drug achieves rarely sustainable results for patients. To overcome this difficulty, the structure of Kenalog was altered by ionizing radiation (IR) to develop a more effective anticancer agent for treatment of various skin cancers. The anticancer effect of modified Kenalog (Kenalog-IR) was assessed in melanoma cancer cells in vitro. The assessment of mitochondrial functions by MTT assay revealed significant inhibition of melanoma cancer cell viability by Kenalog-IR compared to Kenalog. Moreover, Kenalog-IR-induced apoptotic cell death was associated with the intrinsic mitochondrial pathway by triggering the release of intrinsic apoptosis molecules through activation of caspase-related molecules in concentration and time-dependent manners. Furthermore, it was observed that Kenalog-IR-induced apoptosis was associated with the generation of reactive oxygen species (ROS) with increased G2/M cell cycle arrest. Collectively, Kenalog-IR may be a potential suppressor of skin-related cancer in particular melanoma cancer.Abbreviations: IR, ionizing radiation; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; ROS, reactive oxygen species; IMD, incrementally modified drugs

Magnetite nanocrystals draw many attractions for their applications for hyperthermia therapy; cancer killing by heating. These days, hyperthermia together with chemotherapy shows improved cancer treatment effects. So, we tried to synthesize magnetite nanoparticles by thermal decomposition method with different sizes that are important magnetic properties. The size control of magnetite nanoparticles is controlled by changing a molar concentration of iron acetyl acetonate. We also focused on the surface modification of the magnetic nanoparticles with poly ethylene glycol (PEG) to give hydrophilicity and homogeneous dispersion within phosphoric acid-buffer solution (PBS) for the application of hyperthermia. We could present the possibilities of the dispersion of PEG-modified magnetite nanoparticles with different sizes and temperature increase under inductive magnetic field of 6890e.